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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat Potassium voltage-gated channel subfamily S member 3 (Kcns3), partial CSB-YP012097RA1
CSB-EP012097RA1
CSB-BP012097RA1
CSB-MP012097RA1
CSB-EP012097RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Plastin-3 (PLS3), partial CSB-YP018206DXU
CSB-EP018206DXU
CSB-BP018206DXU
CSB-MP018206DXU
CSB-EP018206DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Double-strand-break repair protein rad21 homolog (rad21), partial CSB-YP019257XBE
CSB-EP019257XBE
CSB-BP019257XBE
CSB-MP019257XBE
CSB-EP019257XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trematomus hansoni Hemoglobin subunit beta (hbb) CSB-YP010150TPN
CSB-EP010150TPN
CSB-BP010150TPN
CSB-MP010150TPN
CSB-EP010150TPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Radical S-adenosyl methionine domain-containing protein 2 (rsad2) CSB-YP020536OEI
CSB-EP020536OEI
CSB-BP020536OEI
CSB-MP020536OEI
CSB-EP020536OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica SPARC (SPARC) CSB-YP022486DXJ
CSB-EP022486DXJ
CSB-BP022486DXJ
CSB-MP022486DXJ
CSB-EP022486DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gobionotothen gibberifrons L-lactate dehydrogenase A chain (ldha) CSB-YP012832GGX
CSB-EP012832GGX
CSB-BP012832GGX
CSB-MP012832GGX
CSB-EP012832GGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA polymerase delta subunit 2 (pold2) CSB-YP018304XBE
CSB-EP018304XBE
CSB-BP018304XBE
CSB-MP018304XBE
CSB-EP018304XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus Flap endonuclease 1 (fen) CSB-YP008585FHW
CSB-EP008585FHW
CSB-BP008585FHW
CSB-MP008585FHW
CSB-EP008585FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Geranylgeranyl transferase type-2 subunit beta (BET2) CSB-YP019235CZF
CSB-EP019235CZF
CSB-BP019235CZF
CSB-MP019235CZF
CSB-EP019235CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Glycogen [starch] synthase (7F4.080, NCU06687), partial CSB-YP010079NHA
CSB-EP010079NHA
CSB-BP010079NHA
CSB-MP010079NHA
CSB-EP010079NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Eukaryotic translation initiation factor 3 subunit D (moe1), partial CSB-YP007533SXV
CSB-EP007533SXV
CSB-BP007533SXV
CSB-MP007533SXV
CSB-EP007533SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Exportin-T (los1), partial CSB-YP026226SXV
CSB-EP026226SXV
CSB-BP026226SXV
CSB-MP026226SXV
CSB-EP026226SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA replication complex GINS protein psf2 (psf2) CSB-YP009431SXV
CSB-EP009431SXV
CSB-BP009431SXV
CSB-MP009431SXV
CSB-EP009431SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 21 (med21) CSB-YP013654SXV
CSB-EP013654SXV
CSB-BP013654SXV
CSB-MP013654SXV
CSB-EP013654SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe U6 snRNA-associated Sm-like protein LSm2 (lsm2) CSB-YP013205SXV
CSB-EP013205SXV
CSB-BP013205SXV
CSB-MP013205SXV
CSB-EP013205SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Geranylgeranyl transferase type-2 subunit alpha (bet4) CSB-YP019234SXV
CSB-EP019234SXV
CSB-BP019234SXV
CSB-MP019234SXV
CSB-EP019234SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe COP9 signalosome complex subunit 5 (csn5) CSB-YP005792SXV
CSB-EP005792SXV
CSB-BP005792SXV
CSB-MP005792SXV
CSB-EP005792SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Peroxisomal membrane protein PEX16 (pex16) CSB-YP017801SXV
CSB-EP017801SXV
CSB-BP017801SXV
CSB-MP017801SXV
CSB-EP017801SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 8 (med8) CSB-YP013668SXV
CSB-EP013668SXV
CSB-BP013668SXV
CSB-MP013668SXV
CSB-EP013668SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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