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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pongo pygmaeus Ribonuclease K6 (RNASE6) CSB-YP019797EXP
CSB-EP019797EXP
CSB-BP019797EXP
CSB-MP019797EXP
CSB-EP019797EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Miopithecus talapoin Ribonuclease K6 (RNASE6) CSB-YP019797MUB
CSB-EP019797MUB
CSB-BP019797MUB
CSB-MP019797MUB
CSB-EP019797MUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae ATP synthase subunit a (ATP6), partial CSB-YP015070BVY1
CSB-EP015070BVY1
CSB-BP015070BVY1
CSB-MP015070BVY1
CSB-EP015070BVY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metridium senile Cytochrome c oxidase subunit 3 (COIII), partial CSB-YP015074MTL1
CSB-EP015074MTL1
CSB-BP015074MTL1
CSB-MP015074MTL1
CSB-EP015074MTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepus timidus Cytochrome b (MT-CYB), partial CSB-YP015075LPC
CSB-EP015075LPC
CSB-BP015075LPC
CSB-MP015075LPC
CSB-EP015075LPC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chrysocyon brachyurus Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073DTE1
CSB-EP015073DTE1
CSB-BP015073DTE1
CSB-MP015073DTE1
CSB-EP015073DTE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudalopex gymnocercus Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073EZO1
CSB-EP015073EZO1
CSB-BP015073EZO1
CSB-MP015073EZO1
CSB-EP015073EZO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tragelaphus oryx Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074TPE1
CSB-EP015074TPE1
CSB-BP015074TPE1
CSB-MP015074TPE1
CSB-EP015074TPE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Raphicerus campestris Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074RJQ1
CSB-EP015074RJQ1
CSB-BP015074RJQ1
CSB-MP015074RJQ1
CSB-EP015074RJQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antidorcas marsupialis Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074DNC1
CSB-EP015074DNC1
CSB-BP015074DNC1
CSB-MP015074DNC1
CSB-EP015074DNC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gazella saudiya Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074GFC1
CSB-EP015074GFC1
CSB-BP015074GFC1
CSB-MP015074GFC1
CSB-EP015074GFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Madoqua guentheri Cytochrome b (MT-CYB), partial CSB-YP015075MPK
CSB-EP015075MPK
CSB-BP015075MPK
CSB-MP015075MPK
CSB-EP015075MPK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alligator mississippiensis ATP synthase subunit a (MT-ATP6), partial CSB-YP015070AZO1
CSB-EP015070AZO1
CSB-BP015070AZO1
CSB-MP015070AZO1
CSB-EP015070AZO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nomascus gabriellae Cytochrome b (MT-CYB), partial CSB-YP015075NHL
CSB-EP015075NHL
CSB-BP015075NHL
CSB-MP015075NHL
CSB-EP015075NHL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acrantophis madagascariensis Cytochrome b (MT-CYB), partial CSB-YP015075AXB
CSB-EP015075AXB
CSB-BP015075AXB
CSB-MP015075AXB
CSB-EP015075AXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epicrates angulifer Cytochrome b (MT-CYB), partial CSB-YP015075EMB
CSB-EP015075EMB
CSB-BP015075EMB
CSB-MP015075EMB
CSB-EP015075EMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epicrates striatus Cytochrome b (MT-CYB), partial CSB-YP015075EMG
CSB-EP015075EMG
CSB-BP015075EMG
CSB-MP015075EMG
CSB-EP015075EMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epicrates subflavus Cytochrome b (MT-CYB), partial CSB-YP015075EMK
CSB-EP015075EMK
CSB-BP015075EMK
CSB-MP015075EMK
CSB-EP015075EMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eryx miliaris Cytochrome b (MT-CYB), partial CSB-YP015075ENI
CSB-EP015075ENI
CSB-BP015075ENI
CSB-MP015075ENI
CSB-EP015075ENI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antaresia maculosa Cytochrome b (MT-CYB), partial CSB-YP015075BZR
CSB-EP015075BZR
CSB-BP015075BZR
CSB-MP015075BZR
CSB-EP015075BZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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