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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe Dynein intermediate chain 1 (dic1), partial CSB-YP006997SXV
CSB-EP006997SXV
CSB-BP006997SXV
CSB-MP006997SXV
CSB-EP006997SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitochondrial inner membrane protein cox18 (cox18), partial CSB-YP005830SXV
CSB-EP005830SXV
CSB-BP005830SXV
CSB-MP005830SXV
CSB-EP005830SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA repair protein rev1 (rev1), partial CSB-YP019576SXV
CSB-EP019576SXV
CSB-BP019576SXV
CSB-MP019576SXV
CSB-EP019576SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Dolichol-phosphate mannosyltransferase subunit 3 (dpm3), partial CSB-YP007136SXV1
CSB-EP007136SXV1
CSB-BP007136SXV1
CSB-MP007136SXV1
CSB-EP007136SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Nucleoporin gle1 (gle1) CSB-YP009496SXV
CSB-EP009496SXV
CSB-BP009496SXV
CSB-MP009496SXV
CSB-EP009496SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase III subunit rpc1 (rpc1), partial CSB-YP018343SXV
CSB-EP018343SXV
CSB-BP018343SXV
CSB-MP018343SXV
CSB-EP018343SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Conserved oligomeric Golgi complex subunit 6 (cog6), partial CSB-YP005710SXV
CSB-EP005710SXV
CSB-BP005710SXV
CSB-MP005710SXV
CSB-EP005710SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 8 (taf8) CSB-YP023099SXV
CSB-EP023099SXV
CSB-BP023099SXV
CSB-MP023099SXV
CSB-EP023099SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S18 (rps18a) CSB-YP020385SXV
CSB-EP020385SXV
CSB-BP020385SXV
CSB-MP020385SXV
CSB-EP020385SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (NDUFB4), partial CSB-YP015651HU1
CSB-EP015651HU1
CSB-BP015651HU1
CSB-MP015651HU1
CSB-EP015651HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryctes rhinoceros Defensin CSB-YP006652OFD
CSB-EP006652OFD
CSB-BP006652OFD
CSB-MP006652OFD
CSB-EP006652OFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Arginyl-tRNA--protein transferase 1 (Ate1) CSB-YP002268DLU
CSB-EP002268DLU
CSB-BP002268DLU
CSB-MP002268DLU
CSB-EP002268DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila lebanonensis Aromatic-L-amino-acid decarboxylase (Ddc) CSB-YP006583DLQ
CSB-EP006583DLQ
CSB-BP006583DLQ
CSB-MP006583DLQ
CSB-EP006583DLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Actin-related protein 2/3 complex subunit 3 (arcC) CSB-YP002128DKK
CSB-EP002128DKK
CSB-BP002128DKK
CSB-MP002128DKK
CSB-EP002128DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Actin-related protein 2/3 complex subunit 5 (arcE) CSB-YP002131DKK
CSB-EP002131DKK
CSB-BP002131DKK
CSB-MP002131DKK
CSB-EP002131DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Thymidylate synthase CSB-YP025393DOS
CSB-EP025393DOS
CSB-BP025393DOS
CSB-MP025393DOS
CSB-EP025393DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Encephalitozoon cuniculi Thymidine kinase (TK) CSB-YP023577EKH
CSB-EP023577EKH
CSB-BP023577EKH
CSB-MP023577EKH
CSB-EP023577EKH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Serine/threonine-protein kinase PLK4 (SAK), partial CSB-YP018196DLU
CSB-EP018196DLU
CSB-BP018196DLU
CSB-MP018196DLU
CSB-EP018196DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pescadillo homolog (LmjF22.0030, LmjF_04_0810), partial CSB-YP017791LOG
CSB-EP017791LOG
CSB-BP017791LOG
CSB-MP017791LOG
CSB-EP017791LOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orcinus orca Interleukin-2 (IL2) CSB-YP011629OEP
CSB-EP011629OEP
CSB-BP011629OEP
CSB-MP011629OEP
CSB-EP011629OEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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