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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Borrelia burgdorferi Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717BUD
CSB-EP009717BUD
CSB-BP009717BUD
CSB-MP009717BUD
CSB-EP009717BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Telomeric repeat-binding factor 1 (TERF1) CSB-YP023386DXU
CSB-EP023386DXU
CSB-BP023386DXU
CSB-MP023386DXU
CSB-EP023386DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA polymerase beta (polb) CSB-YP018302XBE
CSB-EP018302XBE
CSB-BP018302XBE
CSB-MP018302XBE
CSB-EP018302XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xiphophorus helleri Cellular tumor antigen p53 (tp53) CSB-YP024077XBJ
CSB-EP024077XBJ
CSB-BP024077XBJ
CSB-MP024077XBJ
CSB-EP024077XBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Surfeit locus protein 4 (surf4), partial CSB-YP022965TKY1
CSB-EP022965TKY1
CSB-BP022965TKY1
CSB-MP022965TKY1
CSB-EP022965TKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phasianus colchicus colchicus Interferon gamma (IFNG) CSB-YP011050EUO
CSB-EP011050EUO
CSB-BP011050EUO
CSB-MP011050EUO
CSB-EP011050EUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Adenosylhomocysteinase (ahcY) CSB-YP001474FHX
CSB-EP001474FHX
CSB-BP001474FHX
CSB-MP001474FHX
CSB-EP001474FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitochondrial Rho GTPase 1 (gem1), partial CSB-YP019695SXV1
CSB-EP019695SXV1
CSB-BP019695SXV1
CSB-MP019695SXV1
CSB-EP019695SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 13 (taf13) CSB-YP023080SXV
CSB-EP023080SXV
CSB-BP023080SXV
CSB-MP023080SXV
CSB-EP023080SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe SRR1-like protein (SPBC14C8.13) CSB-YP022692SXV
CSB-EP022692SXV
CSB-BP022692SXV
CSB-MP022692SXV
CSB-EP022692SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S16 (rps16a) CSB-YP020379SXV
CSB-EP020379SXV
CSB-BP020379SXV
CSB-MP020379SXV
CSB-EP020379SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pescadillo homolog (ppp1), partial CSB-YP017791SXV
CSB-EP017791SXV
CSB-BP017791SXV
CSB-MP017791SXV
CSB-EP017791SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Replication factor C subunit 1 (rfc1), partial CSB-YP019588SXV
CSB-EP019588SXV
CSB-BP019588SXV
CSB-MP019588SXV
CSB-EP019588SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula Somatotropin (GH1) CSB-YP009407TQG
CSB-EP009407TQG
CSB-BP009407TQG
CSB-MP009407TQG
CSB-EP009407TQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica Sperm surface protein Sp17 (SPA17) CSB-YP022451MUE
CSB-EP022451MUE
CSB-BP022451MUE
CSB-MP022451MUE
CSB-EP022451MUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antechinus minimus Cytochrome b (MT-CYB), partial CSB-YP015075BZW
CSB-EP015075BZW
CSB-BP015075BZW
CSB-MP015075BZW
CSB-EP015075BZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tapirus terrestris Cytochrome b (MT-CYB), partial CSB-YP015075TLG
CSB-EP015075TLG
CSB-BP015075TLG
CSB-MP015075TLG
CSB-EP015075TLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas penelope NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077BZC
CSB-EP015077BZC
CSB-BP015077BZC
CSB-MP015077BZC
CSB-EP015077BZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glis glis ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071GGH1
CSB-EP015071GGH1
CSB-BP015071GGH1
CSB-MP015071GGH1
CSB-EP015071GGH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxisomal acyl-coenzyme A oxidase 1 (ACX1), partial CSB-YP001172DOA
CSB-EP001172DOA
CSB-BP001172DOA
CSB-MP001172DOA
CSB-EP001172DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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