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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vulpes macrotis Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073VFV1
CSB-EP015073VFV1
CSB-BP015073VFV1
CSB-MP015073VFV1
CSB-EP015073VFV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tragelaphus strepsiceros Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074TPH1
CSB-EP015074TPH1
CSB-BP015074TPH1
CSB-MP015074TPH1
CSB-EP015074TPH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cephalophus natalensis Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074DRE1
CSB-EP015074DRE1
CSB-BP015074DRE1
CSB-MP015074DRE1
CSB-EP015074DRE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litocranius walleri Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074LQC1
CSB-EP015074LQC1
CSB-BP015074LQC1
CSB-MP015074LQC1
CSB-EP015074LQC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Raphicerus campestris Cytochrome b (MT-CYB), partial CSB-YP015075RJQ
CSB-EP015075RJQ
CSB-BP015075RJQ
CSB-MP015075RJQ
CSB-EP015075RJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alligator mississippiensis NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076AZO
CSB-EP015076AZO
CSB-BP015076AZO
CSB-MP015076AZO
CSB-EP015076AZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capreolus pygargus Cytochrome b (MT-CYB), partial CSB-YP015075DQC
CSB-EP015075DQC
CSB-BP015075DQC
CSB-MP015075DQC
CSB-EP015075DQC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acrantophis dumerili Cytochrome b (MT-CYB), partial CSB-YP015075AXA
CSB-EP015075AXA
CSB-BP015075AXA
CSB-MP015075AXA
CSB-EP015075AXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Casarea dussumieri Cytochrome b (MT-CYB), partial CSB-YP015075DQJ
CSB-EP015075DQJ
CSB-BP015075DQJ
CSB-MP015075DQJ
CSB-EP015075DQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eunectes notaeus Cytochrome b (MT-CYB), partial CSB-YP015075EPK
CSB-EP015075EPK
CSB-BP015075EPK
CSB-MP015075EPK
CSB-EP015075EPK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eryx jaculus Cytochrome b (MT-CYB), partial CSB-YP015075ENH
CSB-EP015075ENH
CSB-BP015075ENH
CSB-MP015075ENH
CSB-EP015075ENH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiopython albertisii Cytochrome b (MT-CYB), partial CSB-YP015075LOA
CSB-EP015075LOA
CSB-BP015075LOA
CSB-MP015075LOA
CSB-EP015075LOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxocemus bicolor Cytochrome b (MT-CYB), partial CSB-YP015075LQR
CSB-EP015075LQR
CSB-BP015075LQR
CSB-MP015075LQR
CSB-EP015075LQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sanzinia madagascariensis Cytochrome b (MT-CYB), partial CSB-YP015075SXG
CSB-EP015075SXG
CSB-BP015075SXG
CSB-MP015075SXG
CSB-EP015075SXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gazella subgutturosa Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074GFD1
CSB-EP015074GFD1
CSB-BP015074GFD1
CSB-MP015074GFD1
CSB-EP015074GFD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 60S ribosomal protein L17 (RPL17) CSB-YP020149ZAX
CSB-EP020149ZAX
CSB-BP020149ZAX
CSB-MP020149ZAX
CSB-EP020149ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus luteus 60S ribosomal protein L30 (RPL30) CSB-YP020225LRK
CSB-EP020225LRK
CSB-BP020225LRK
CSB-MP020225LRK
CSB-EP020225LRK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Elongation factor Tu (tuf) CSB-YP025342GFM
CSB-EP025342GFM
CSB-BP025342GFM
CSB-MP025342GFM
CSB-EP025342GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Peptide deformylase (def) CSB-YP017707BUD
CSB-EP017707BUD
CSB-BP017707BUD
CSB-MP017707BUD
CSB-EP017707BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Elongation factor Ts (tsf) CSB-YP025124BUD
CSB-EP025124BUD
CSB-BP025124BUD
CSB-MP025124BUD
CSB-EP025124BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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