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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Branchiostoma lanceolatum NADH-ubiquinone oxidoreductase chain 4 (ND4), partial CSB-YP015079BVZ
CSB-EP015079BVZ
CSB-BP015079BVZ
CSB-MP015079BVZ
CSB-EP015079BVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex arcticus Cytochrome b (MT-CYB), partial CSB-YP015075FIP
CSB-EP015075FIP
CSB-BP015075FIP
CSB-MP015075FIP
CSB-EP015075FIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex haydeni Cytochrome b (MT-CYB), partial CSB-YP015075FIZ
CSB-EP015075FIZ
CSB-BP015075FIZ
CSB-MP015075FIZ
CSB-EP015075FIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex raddei Cytochrome b (MT-CYB), partial CSB-YP015075FJJ
CSB-EP015075FJJ
CSB-BP015075FJJ
CSB-MP015075FJJ
CSB-EP015075FJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Harpactes diardii Cytochrome b (MT-CYB), partial CSB-YP015075HUM
CSB-EP015075HUM
CSB-BP015075HUM
CSB-MP015075HUM
CSB-EP015075HUM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinodon semicarinatum ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071DKP
CSB-EP015071DKP
CSB-BP015071DKP
CSB-MP015071DKP
CSB-EP015071DKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinodon semicarinatum NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079DKP
CSB-EP015079DKP
CSB-BP015079DKP
CSB-MP015079DKP
CSB-EP015079DKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyplectron bicalcaratum Cytochrome b (MT-CYB), partial CSB-YP015075EXN
CSB-EP015075EXN
CSB-BP015075EXN
CSB-MP015075EXN
CSB-EP015075EXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelomedusa subrufa Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073ETC1
CSB-EP015073ETC1
CSB-BP015073ETC1
CSB-MP015073ETC1
CSB-EP015073ETC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelomedusa subrufa NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081ETC
CSB-EP015081ETC
CSB-BP015081ETC
CSB-MP015081ETC
CSB-EP015081ETC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vireo flavifrons Cytochrome b (MT-CYB), partial CSB-YP015075VFP
CSB-EP015075VFP
CSB-BP015075VFP
CSB-MP015075VFP
CSB-EP015075VFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex samniticus Cytochrome b (MT-CYB), partial CSB-YP015075FJM
CSB-EP015075FJM
CSB-BP015075FJM
CSB-MP015075FJM
CSB-EP015075FJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex granarius Cytochrome b (MT-CYB), partial CSB-YP015075FIY
CSB-EP015075FIY
CSB-BP015075FIY
CSB-MP015075FIY
CSB-EP015075FIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorex isodon Cytochrome b (MT-CYB), partial CSB-YP015075FJC
CSB-EP015075FJC
CSB-BP015075FJC
CSB-MP015075FJC
CSB-EP015075FJC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana sylvestris S-adenosylmethionine decarboxylase proenzyme (SAMDC1) CSB-YP001658NHD
CSB-EP001658NHD
CSB-BP001658NHD
CSB-MP001658NHD
CSB-EP001658NHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Villin-1 (VLN1), partial CSB-YP025855DOA
CSB-EP025855DOA
CSB-BP025855DOA
CSB-MP025855DOA
CSB-EP025855DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petunia hybrida 60S ribosomal protein L15 (RPL15) CSB-YP020144EUD
CSB-EP020144EUD
CSB-BP020144EUD
CSB-MP020144EUD
CSB-EP020144EUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L21 (RPL21) CSB-YP020163DZX
CSB-EP020163DZX
CSB-BP020163DZX
CSB-MP020163DZX
CSB-EP020163DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum V-type proton ATPase subunit G 1 (VATG1) CSB-YP002408NHE
CSB-EP002408NHE
CSB-BP002408NHE
CSB-MP002408NHE
CSB-EP002408NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Quercus suber 60S ribosomal protein L41 (RPL41) CSB-YP020290QAC
CSB-EP020290QAC
CSB-BP020290QAC
CSB-MP020290QAC
CSB-EP020290QAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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