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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhipicephalus sanguineus NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078RKI1
CSB-EP015078RKI1
CSB-BP015078RKI1
CSB-MP015078RKI1
CSB-EP015078RKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus NADH-ubiquinone oxidoreductase chain 6 (ND6), partial CSB-YP015082RKI1
CSB-EP015082RKI1
CSB-BP015082RKI1
CSB-MP015082RKI1
CSB-EP015082RKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe AP-1 complex subunit beta-1 (apl2), partial CSB-YP001860SXV
CSB-EP001860SXV
CSB-BP001860SXV
CSB-MP001860SXV
CSB-EP001860SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 40S ribosomal protein S7 (rps-7) CSB-YP020476NHA
CSB-EP020476NHA
CSB-BP020476NHA
CSB-MP020476NHA
CSB-EP020476NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eimeria tenella 40S ribosomal protein S3a CSB-YP020444EJU
CSB-EP020444EJU
CSB-BP020444EJU
CSB-MP020444EJU
CSB-EP020444EJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichuris trichiura Tubulin beta chain CSB-YP025318TQH
CSB-EP025318TQH
CSB-BP025318TQH
CSB-MP025318TQH
CSB-EP025318TQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA-processing protein 8 (T07A9.8) CSB-YP020531CXY
CSB-EP020531CXY
CSB-BP020531CXY
CSB-MP020531CXY
CSB-EP020531CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum Fatty acid-binding protein CSB-YP007956SXP
CSB-EP007956SXP
CSB-BP007956SXP
CSB-MP007956SXP
CSB-EP007956SXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori TATA-box-binding protein (Tbp) CSB-YP023239BTT
CSB-EP023239BTT
CSB-BP023239BTT
CSB-MP023239BTT
CSB-EP023239BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula Follitropin subunit beta (FSHB) CSB-YP009018TQG
CSB-EP009018TQG
CSB-BP009018TQG
CSB-MP009018TQG
CSB-EP009018TQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiri sciureus Ribonuclease K6 (RNASE6) CSB-YP019797SWD
CSB-EP019797SWD
CSB-BP019797SWD
CSB-MP019797SWD
CSB-EP019797SWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Amiloride-sensitive sodium channel subunit delta (SCNN1D), partial CSB-YP020850EQV
CSB-EP020850EQV
CSB-BP020850EQV
CSB-MP020850EQV
CSB-EP020850EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equus burchelli Glycoprotein hormones alpha chain (CGA) CSB-YP005293EMO
CSB-EP005293EMO
CSB-BP005293EMO
CSB-MP005293EMO
CSB-EP005293EMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beatragus hunteri Cytochrome b (MT-CYB), partial CSB-YP015075BSM
CSB-EP015075BSM
CSB-BP015075BSM
CSB-MP015075BSM
CSB-EP015075BSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae Cytochrome b (MT-CYB), partial CSB-YP015075BVY
CSB-EP015075BVY
CSB-BP015075BVY
CSB-MP015075BVY
CSB-EP015075BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metridium senile NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080MTL1
CSB-EP015080MTL1
CSB-BP015080MTL1
CSB-MP015080MTL1
CSB-EP015080MTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metridium senile Cytochrome b (MT-CYB), partial CSB-YP015075MTL
CSB-EP015075MTL
CSB-BP015075MTL
CSB-MP015075MTL
CSB-EP015075MTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepus californicus Cytochrome b (MT-CYB), partial CSB-YP015075LOY
CSB-EP015075LOY
CSB-BP015075LOY
CSB-MP015075LOY
CSB-EP015075LOY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moschus leucogaster Cytochrome b (MT-CYB), partial CSB-YP015075MUO
CSB-EP015075MUO
CSB-BP015075MUO
CSB-MP015075MUO
CSB-EP015075MUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes zerda Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073VFX1
CSB-EP015073VFX1
CSB-BP015073VFX1
CSB-MP015073VFX1
CSB-EP015073VFX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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