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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Prunus armeniaca 40S ribosomal protein S4 (RPS4) CSB-YP020458EZH
CSB-EP020458EZH
CSB-BP020458EZH
CSB-MP020458EZH
CSB-EP020458EZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta vulgaris Elongation factor 1-delta CSB-YP007431BSQ
CSB-EP007431BSQ
CSB-BP007431BSQ
CSB-MP007431BSQ
CSB-EP007431BSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petunia hybrida Proteasome subunit alpha type-4 (PAC1) CSB-YP018869EUD
CSB-EP018869EUD
CSB-BP018869EUD
CSB-MP018869EUD
CSB-EP018869EUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Quercus suber 60S ribosomal protein L15 (RPL15) CSB-YP020144QAC
CSB-EP020144QAC
CSB-BP020144QAC
CSB-MP020144QAC
CSB-EP020144QAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase (glpD), partial CSB-YP009711FGQ
CSB-EP009711FGQ
CSB-BP009711FGQ
CSB-MP009711FGQ
CSB-EP009711FGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Acyl carrier protein (acpP) CSB-YP015636TPR
CSB-EP015636TPR
CSB-BP015636TPR
CSB-MP015636TPR
CSB-EP015636TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum tRNA pseudouridine synthase A (truA) CSB-YP019086TPR
CSB-EP019086TPR
CSB-BP019086TPR
CSB-MP019086TPR
CSB-EP019086TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Translation initiation factor IF-3 (infC) CSB-YP015166TPR
CSB-EP015166TPR
CSB-BP015166TPR
CSB-MP015166TPR
CSB-EP015166TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Translation initiation factor IF-2 (infB), partial CSB-YP015164TPR
CSB-EP015164TPR
CSB-BP015164TPR
CSB-MP015164TPR
CSB-EP015164TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Nucleoside diphosphate kinase (ndk) CSB-YP015889TPR
CSB-EP015889TPR
CSB-BP015889TPR
CSB-MP015889TPR
CSB-EP015889TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Superoxide dismutase [Mn] (sodA) CSB-YP022398DSB
CSB-EP022398DSB
CSB-BP022398DSB
CSB-MP022398DSB
CSB-EP022398DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Triosephosphate isomerase (tpiA) CSB-YP024102DSB
CSB-EP024102DSB
CSB-BP024102DSB
CSB-MP024102DSB
CSB-EP024102DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Nucleoside diphosphate kinase (ndk) CSB-YP015889MVX
CSB-EP015889MVX
CSB-BP015889MVX
CSB-MP015889MVX
CSB-EP015889MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transfer flavoprotein subunit beta (etfB) CSB-YP007845MQR
CSB-EP007845MQR
CSB-BP007845MQR
CSB-MP007845MQR
CSB-EP007845MQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MVP
CSB-EP022398MVP
CSB-BP022398MVP
CSB-MP022398MVP
CSB-EP022398MVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Branched-chain-amino-acid aminotransferase (ilvE) CSB-YP002601EZX
CSB-EP002601EZX
CSB-BP002601EZX
CSB-MP002601EZX
CSB-EP002601EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor tRNA pseudouridine synthase A (truA) CSB-YP019086FOB
CSB-EP019086FOB
CSB-BP019086FOB
CSB-MP019086FOB
CSB-EP019086FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Metaxin-2 (Mtx2) CSB-YP015213MO
CSB-EP015213MO
CSB-BP015213MO
CSB-MP015213MO
CSB-EP015213MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL), partial CSB-YP002123MRG
CSB-EP002123MRG
CSB-BP002123MRG
CSB-MP002123MRG
CSB-EP002123MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Origin recognition complex subunit 4 (Orc4) CSB-YP017234MO
CSB-EP017234MO
CSB-BP017234MO
CSB-MP017234MO
CSB-EP017234MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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