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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Borrelia burgdorferi Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BUD
CSB-EP015150BUD
CSB-BP015150BUD
CSB-MP015150BUD
CSB-EP015150BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Ribonuclease 3 (rnc) CSB-YP019809BUD
CSB-EP019809BUD
CSB-BP019809BUD
CSB-MP019809BUD
CSB-EP019809BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum ATP synthase subunit alpha (atpA), partial CSB-YP002344BXE
CSB-EP002344BXE
CSB-BP002344BXE
CSB-MP002344BXE
CSB-EP002344BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus megaterium Beta-galactosidase (bgaM), partial CSB-YP009476BQX
CSB-EP009476BQX
CSB-BP009476BQX
CSB-MP009476BQX
CSB-EP009476BQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidine kinase (tdk) CSB-YP023577BRL
CSB-EP023577BRL
CSB-BP023577BRL
CSB-MP023577BRL
CSB-EP023577BRL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti UDP-glucose 6-dehydrogenase (rkpK) CSB-YP025567RKU
CSB-EP025567RKU
CSB-BP025567RKU
CSB-MP025567RKU
CSB-EP025567RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger protein ZIC 3 (zic3) CSB-YP026486XBE
CSB-EP026486XBE
CSB-BP026486XBE
CSB-MP026486XBE
CSB-EP026486XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Rho GTPase-activating protein 6 (arhgap6) CSB-YP002034TKY
CSB-EP002034TKY
CSB-BP002034TKY
CSB-MP002034TKY
CSB-EP002034TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana Voltage-dependent L-type calcium channel subunit alpha-1S, partial CSB-YP004406LQA
CSB-EP004406LQA
CSB-BP004406LQA
CSB-MP004406LQA
CSB-EP004406LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Splicing factor 3B subunit 1 (sf3b1), partial CSB-YP021125XBE
CSB-EP021125XBE
CSB-BP021125XBE
CSB-MP021125XBE
CSB-EP021125XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii DNA primase large subunit (priB) CSB-YP018681FHX
CSB-EP018681FHX
CSB-BP018681FHX
CSB-MP018681FHX
CSB-EP018681FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614FHX
CSB-EP017614FHX
CSB-BP017614FHX
CSB-MP017614FHX
CSB-EP017614FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Serine hydroxymethyltransferase (glyA) CSB-YP021274FHX
CSB-EP021274FHX
CSB-BP021274FHX
CSB-MP021274FHX
CSB-EP021274FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Methylated-DNA--protein-cysteine methyltransferase (ogt) CSB-YP013788FHX
CSB-EP013788FHX
CSB-BP013788FHX
CSB-MP013788FHX
CSB-EP013788FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Inorganic pyrophosphatase (ppa) CSB-YP018410FHX
CSB-EP018410FHX
CSB-BP018410FHX
CSB-MP018410FHX
CSB-EP018410FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 7 (med7) CSB-YP013667SXV
CSB-EP013667SXV
CSB-BP013667SXV
CSB-MP013667SXV
CSB-EP013667SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Long-chain-fatty-acid--CoA ligase 1 (lcf1), partial CSB-YP001191SXV
CSB-EP001191SXV
CSB-BP001191SXV
CSB-MP001191SXV
CSB-EP001191SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Methylthioribose-1-phosphate isomerase (mri1) CSB-YP014799SXV
CSB-EP014799SXV
CSB-BP014799SXV
CSB-MP014799SXV
CSB-EP014799SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptochiton stelleri 60S ribosomal protein L37a (RPL37A) CSB-YP020267DYK
CSB-EP020267DYK
CSB-BP020267DYK
CSB-MP020267DYK
CSB-EP020267DYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles stephensi Nitric oxide synthase, partial CSB-YP015946BZM
CSB-EP015946BZM
CSB-BP015946BZM
CSB-MP015946BZM
CSB-EP015946BZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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