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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Cysteine desulfurase (iscS) CSB-YP015769ENX
CSB-EP015769ENX
CSB-BP015769ENX
CSB-MP015769ENX
CSB-EP015769ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-ketoacyl-CoA thiolase (fadI) CSB-YP001117ENX
CSB-EP001117ENX
CSB-BP001117ENX
CSB-MP001117ENX
CSB-EP001117ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA pseudouridine synthase A (truA) CSB-YP019086ENX
CSB-EP019086ENX
CSB-BP019086ENX
CSB-MP019086ENX
CSB-EP019086ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipoyl synthase (lipA) CSB-YP012927ENX
CSB-EP012927ENX
CSB-BP012927ENX
CSB-MP012927ENX
CSB-EP012927ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415ENX
CSB-EP015415ENX
CSB-BP015415ENX
CSB-MP015415ENX
CSB-EP015415ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. Galactokinase (galK) CSB-YP009198TNM
CSB-EP009198TNM
CSB-BP009198TNM
CSB-MP009198TNM
CSB-EP009198TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. Elongation factor Ts (tsf) CSB-YP025124TNM
CSB-EP025124TNM
CSB-BP025124TNM
CSB-MP025124TNM
CSB-EP025124TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Korarchaeum cryptofilum Elongation factor 2 (fusA), partial CSB-YP007434KBQ
CSB-EP007434KBQ
CSB-BP007434KBQ
CSB-MP007434KBQ
CSB-EP007434KBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Korarchaeum cryptofilum Flap endonuclease 1 (fen) CSB-YP008585KBQ
CSB-EP008585KBQ
CSB-BP008585KBQ
CSB-MP008585KBQ
CSB-EP008585KBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Korarchaeum cryptofilum Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824KBQ
CSB-EP005824KBQ
CSB-BP005824KBQ
CSB-MP005824KBQ
CSB-EP005824KBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Korarchaeum cryptofilum DNA ligase 1 (lig1), partial CSB-YP012930KBQ
CSB-EP012930KBQ
CSB-BP012930KBQ
CSB-MP012930KBQ
CSB-EP012930KBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor Ts (tsf) CSB-YP025124DUI
CSB-EP025124DUI
CSB-BP025124DUI
CSB-MP025124DUI
CSB-EP025124DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit delta (atpH) CSB-YP002355DUI
CSB-EP002355DUI
CSB-BP002355DUI
CSB-MP002355DUI
CSB-EP002355DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Translation initiation factor IF-2 (infB), partial CSB-YP015164SYY
CSB-EP015164SYY
CSB-BP015164SYY
CSB-MP015164SYY
CSB-EP015164SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Porphobilinogen deaminase (hemC) CSB-YP010524SYY
CSB-EP010524SYY
CSB-BP010524SYY
CSB-MP010524SYY
CSB-EP010524SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi ATP synthase subunit beta (atpD) CSB-YP002350SYY
CSB-EP002350SYY
CSB-BP002350SYY
CSB-MP002350SYY
CSB-EP002350SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582SYY
CSB-EP009582SYY
CSB-BP009582SYY
CSB-MP009582SYY
CSB-EP009582SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Serine hydroxymethyltransferase (glyA) CSB-YP021274SYY
CSB-EP021274SYY
CSB-BP021274SYY
CSB-MP021274SYY
CSB-EP021274SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Malate dehydrogenase (mdh) CSB-YP013623SYY
CSB-EP013623SYY
CSB-BP013623SYY
CSB-MP013623SYY
CSB-EP013623SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Xaa-Pro dipeptidase (pepQ) CSB-YP017784SYY
CSB-EP017784SYY
CSB-BP017784SYY
CSB-MP017784SYY
CSB-EP017784SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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