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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cenocepacia Acyl carrier protein (acpP) CSB-YP015636BXQ
CSB-EP015636BXQ
CSB-BP015636BXQ
CSB-MP015636BXQ
CSB-EP015636BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451BXQ
CSB-EP015451BXQ
CSB-BP015451BXQ
CSB-MP015451BXQ
CSB-EP015451BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia ATP synthase subunit beta (atpD) CSB-YP002350BXQ
CSB-EP002350BXQ
CSB-BP002350BXQ
CSB-MP002350BXQ
CSB-EP002350BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 NAD-dependent malic enzyme (maeA), partial CSB-YP013633YAX
CSB-EP013633YAX
CSB-BP013633YAX
CSB-MP013633YAX
CSB-EP013633YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Glycine cleavage system H protein (gcvH) CSB-YP009335YAX
CSB-EP009335YAX
CSB-BP009335YAX
CSB-MP009335YAX
CSB-EP009335YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Translation initiation factor IF-2 (infB), partial CSB-YP015164YAX
CSB-EP015164YAX
CSB-BP015164YAX
CSB-MP015164YAX
CSB-EP015164YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415YAX
CSB-EP015415YAX
CSB-BP015415YAX
CSB-MP015415YAX
CSB-EP015415YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP synthase subunit delta (atpH) CSB-YP002355FGC
CSB-EP002355FGC
CSB-BP002355FGC
CSB-MP002355FGC
CSB-EP002355FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Peptide chain release factor 1 (prfA) CSB-YP015197FGC
CSB-EP015197FGC
CSB-BP015197FGC
CSB-MP015197FGC
CSB-EP015197FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Elongation factor Ts (tsf) CSB-YP025124FGC
CSB-EP025124FGC
CSB-BP025124FGC
CSB-MP025124FGC
CSB-EP025124FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917FGC
CSB-EP022917FGC
CSB-BP022917FGC
CSB-MP022917FGC
CSB-EP022917FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Translation initiation factor IF-3 (infC) CSB-YP015166FGC
CSB-EP015166FGC
CSB-BP015166FGC
CSB-MP015166FGC
CSB-EP015166FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Transaldolase (tal) CSB-YP023112FGC
CSB-EP023112FGC
CSB-BP023112FGC
CSB-MP023112FGC
CSB-EP023112FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493ENT
CSB-EP009493ENT
CSB-BP009493ENT
CSB-MP009493ENT
CSB-EP009493ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA dimethylallyltransferase (miaA) CSB-YP024529ENT
CSB-EP024529ENT
CSB-BP024529ENT
CSB-MP024529ENT
CSB-EP024529ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Triosephosphate isomerase (tpiA) CSB-YP024102ENT
CSB-EP024102ENT
CSB-BP024102ENT
CSB-MP024102ENT
CSB-EP024102ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cardiolipin synthase (cls), partial CSB-YP005985ENT1
CSB-EP005985ENT1
CSB-BP005985ENT1
CSB-MP005985ENT1
CSB-EP005985ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415ENT
CSB-EP015415ENT
CSB-BP015415ENT
CSB-MP015415ENT
CSB-EP015415ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA pseudouridine synthase A (truA) CSB-YP019086ENT
CSB-EP019086ENT
CSB-BP019086ENT
CSB-MP019086ENT
CSB-EP019086ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917ENT
CSB-EP022917ENT
CSB-BP022917ENT
CSB-MP022917ENT
CSB-EP022917ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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