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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Tryptophan--tRNA ligase (trpS) CSB-YP300048SKX
CSB-EP300048SKX
CSB-BP300048SKX
CSB-MP300048SKX
CSB-EP300048SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0267 protein yqfB (yqfB) CSB-YP300049EOD
CSB-EP300049EOD
CSB-BP300049EOD
CSB-MP300049EOD
CSB-EP300049EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase (tyrS) CSB-YP300050MVZ
CSB-EP300050MVZ
CSB-BP300050MVZ
CSB-MP300050MVZ
CSB-EP300050MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable Fe (2+)-trafficking protein CSB-YP300051SWW
CSB-EP300051SWW
CSB-BP300051SWW
CSB-MP300051SWW
CSB-EP300051SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0270 protein YheU (yheU) CSB-YP300052EGX
CSB-EP300052EGX
CSB-BP300052EGX
CSB-MP300052EGX
CSB-EP300052EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine recombinase XerC (xerC) CSB-YP300053SKY
CSB-EP300053SKY
CSB-BP300053SKY
CSB-MP300053SKY
CSB-EP300053SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0735 ACT domain-containing protein SAV1643 (SAV1643) CSB-YP300054SKX
CSB-EP300054SKX
CSB-BP300054SKX
CSB-MP300054SKX
CSB-EP300054SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate reductase subunit D (frdD), partial CSB-YP300055MVH1
CSB-EP300055MVH1
CSB-BP300055MVH1
CSB-MP300055MVH1
CSB-EP300055MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulator of ribonuclease activity A (rraA) CSB-YP300056SWW
CSB-EP300056SWW
CSB-BP300056SWW
CSB-MP300056SWW
CSB-EP300056SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional activator AaeR (aaeR) CSB-YP300057EGX
CSB-EP300057EGX
CSB-BP300057EGX
CSB-MP300057EGX
CSB-EP300057EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv1830/MT1879 (Rv1830, MT1879) CSB-YP300058MVZ
CSB-EP300058MVZ
CSB-BP300058MVZ
CSB-MP300058MVZ
CSB-EP300058MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP300059BUA
CSB-EP300059BUA
CSB-BP300059BUA
CSB-MP300059BUA
CSB-EP300059BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP300060SMP
CSB-EP300060SMP
CSB-BP300060SMP
CSB-MP300060SMP
CSB-EP300060SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0289 protein BPP3960 (BPP3960) CSB-YP300061BIK
CSB-EP300061BIK
CSB-BP300061BIK
CSB-MP300061BIK
CSB-EP300061BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator YddM (yddM) CSB-YP300062EGX
CSB-EP300062EGX
CSB-BP300062EGX
CSB-MP300062EGX
CSB-EP300062EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Transcriptional regulator MntR (mntR) CSB-YP300063SXB
CSB-EP300063SXB
CSB-BP300063SXB
CSB-MP300063SXB
CSB-EP300063SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease subunit gamma (ureA) CSB-YP300064SUL
CSB-EP300064SUL
CSB-BP300064SUL
CSB-MP300064SUL
CSB-EP300064SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ion-transport protein yfeO (yfeO), partial CSB-YP300065EOD
CSB-EP300065EOD
CSB-BP300065EOD
CSB-MP300065EOD
CSB-EP300065EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulator NanR (nanR) CSB-YP300066SWW
CSB-EP300066SWW
CSB-BP300066SWW
CSB-MP300066SWW
CSB-EP300066SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv0880/MT0903.1 (Rv0880, MT0903.1) CSB-YP300067MVZ
CSB-EP300067MVZ
CSB-BP300067MVZ
CSB-MP300067MVZ
CSB-EP300067MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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