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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cyanothece sp. Acyl carrier protein (acpP) CSB-YP015636DZY
CSB-EP015636DZY
CSB-BP015636DZY
CSB-MP015636DZY
CSB-EP015636DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Elongation factor Tu (tuf) CSB-YP025342DZY
CSB-EP025342DZY
CSB-BP025342DZY
CSB-MP025342DZY
CSB-EP025342DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Serine hydroxymethyltransferase (glyA) CSB-YP021274DZY
CSB-EP021274DZY
CSB-BP021274DZY
CSB-MP021274DZY
CSB-EP021274DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Cytosolic phospholipase A2 (pla2g4a), partial CSB-YP018097XBF
CSB-EP018097XBF
CSB-BP018097XBF
CSB-MP018097XBF
CSB-EP018097XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein kinase greatwall (mastl), partial CSB-YP013515XBF
CSB-EP013515XBF
CSB-BP013515XBF
CSB-MP013515XBF
CSB-EP013515XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Elongation factor G (fusA), partial CSB-YP009373FOF
CSB-EP009373FOF
CSB-BP009373FOF
CSB-MP009373FOF
CSB-EP009373FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus ATP synthase subunit beta (atpD) CSB-YP002350FOF
CSB-EP002350FOF
CSB-BP002350FOF
CSB-MP002350FOF
CSB-EP002350FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Lipoyl synthase (lipA) CSB-YP012927FOF
CSB-EP012927FOF
CSB-BP012927FOF
CSB-MP012927FOF
CSB-EP012927FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus tRNA dimethylallyltransferase (miaA) CSB-YP024529FOF
CSB-EP024529FOF
CSB-BP024529FOF
CSB-MP024529FOF
CSB-EP024529FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus L-threonine 3-dehydrogenase (tdh) CSB-YP023350FOF
CSB-EP023350FOF
CSB-BP023350FOF
CSB-MP023350FOF
CSB-EP023350FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium urealyticum Nucleoside diphosphate kinase (ndk) CSB-YP015889DXD
CSB-EP015889DXD
CSB-BP015889DXD
CSB-MP015889DXD
CSB-EP015889DXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium urealyticum Elongation factor Tu (tuf) CSB-YP025342DXD
CSB-EP025342DXD
CSB-BP025342DXD
CSB-MP025342DXD
CSB-EP025342DXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase (miaA) CSB-YP024529EVG
CSB-EP024529EVG
CSB-BP024529EVG
CSB-MP024529EVG
CSB-EP024529EVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP015197EVG
CSB-EP015197EVG
CSB-BP015197EVG
CSB-MP015197EVG
CSB-EP015197EVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc citreum Ribosome-recycling factor (frr) CSB-YP014941LPD
CSB-EP014941LPD
CSB-BP014941LPD
CSB-MP014941LPD
CSB-EP014941LPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc citreum GTPase Era (era) CSB-YP007759LPD
CSB-EP007759LPD
CSB-BP007759LPD
CSB-MP007759LPD
CSB-EP007759LPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callicebus moloch Ubiquitin-like protein 4A (UBL4A) CSB-YP025499CYE
CSB-EP025499CYE
CSB-BP025499CYE
CSB-MP025499CYE
CSB-EP025499CYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium abscessus Peptide chain release factor 1 (prfA) CSB-YP015197MVF
CSB-EP015197MVF
CSB-BP015197MVF
CSB-MP015197MVF
CSB-EP015197MVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium abscessus Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033MVF
CSB-EP018033MVF
CSB-BP018033MVF
CSB-MP018033MVF
CSB-EP018033MVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium abscessus Ribosome-recycling factor (frr) CSB-YP014941MVF
CSB-EP014941MVF
CSB-BP014941MVF
CSB-MP014941MVF
CSB-EP014941MVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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