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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methylobacterium radiotolerans Malate dehydrogenase (mdh) CSB-YP013623MTD
CSB-EP013623MTD
CSB-BP013623MTD
CSB-MP013623MTD
CSB-EP013623MTD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033ENX
CSB-EP018033ENX
CSB-BP018033ENX
CSB-MP018033ENX
CSB-EP018033ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucose-6-phosphate isomerase (pgi) CSB-YP009717ENX
CSB-EP009717ENX
CSB-BP009717ENX
CSB-MP009717ENX
CSB-EP009717ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucokinase (glk) CSB-YP009319ENX
CSB-EP009319ENX
CSB-BP009319ENX
CSB-MP009319ENX
CSB-EP009319ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Galactokinase (galK) CSB-YP009198ENX
CSB-EP009198ENX
CSB-BP009198ENX
CSB-MP009198ENX
CSB-EP009198ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide deformylase (def) CSB-YP017707ENX
CSB-EP017707ENX
CSB-BP017707ENX
CSB-MP017707ENX
CSB-EP017707ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycine cleavage system H protein (gcvH) CSB-YP009335ENX
CSB-EP009335ENX
CSB-BP009335ENX
CSB-MP009335ENX
CSB-EP009335ENX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. Ribosome-recycling factor (frr) CSB-YP014941TNM
CSB-EP014941TNM
CSB-BP014941TNM
CSB-MP014941TNM
CSB-EP014941TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344TNM
CSB-EP002344TNM
CSB-BP002344TNM
CSB-MP002344TNM
CSB-EP002344TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Korarchaeum cryptofilum Protein pelota homolog (pelA) CSB-YP017778KBQ
CSB-EP017778KBQ
CSB-BP017778KBQ
CSB-MP017778KBQ
CSB-EP017778KBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase Era (era) CSB-YP007759DUI
CSB-EP007759DUI
CSB-BP007759DUI
CSB-MP007759DUI
CSB-EP007759DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor G (fusA), partial CSB-YP009373DUI
CSB-EP009373DUI
CSB-BP009373DUI
CSB-MP009373DUI
CSB-EP009373DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Urocanate hydratase (hutU), partial CSB-YP025677SYY
CSB-EP025677SYY
CSB-BP025677SYY
CSB-MP025677SYY
CSB-EP025677SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi L-threonine 3-dehydrogenase (tdh) CSB-YP023350SYY
CSB-EP023350SYY
CSB-BP023350SYY
CSB-MP023350SYY
CSB-EP023350SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi tRNA dimethylallyltransferase (miaA) CSB-YP024529SYY
CSB-EP024529SYY
CSB-BP024529SYY
CSB-MP024529SYY
CSB-EP024529SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SYY
CSB-EP015150SYY
CSB-BP015150SYY
CSB-MP015150SYY
CSB-EP015150SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Porphobilinogen deaminase (hemC) CSB-YP010524BXQ
CSB-EP010524BXQ
CSB-BP010524BXQ
CSB-MP010524BXQ
CSB-EP010524BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Transaldolase (tal) CSB-YP023112BXQ
CSB-EP023112BXQ
CSB-BP023112BXQ
CSB-MP023112BXQ
CSB-EP023112BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Nucleoside diphosphate kinase (ndk) CSB-YP015889BXQ
CSB-EP015889BXQ
CSB-BP015889BXQ
CSB-MP015889BXQ
CSB-EP015889BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Ribosome-recycling factor (frr) CSB-YP014941BXQ
CSB-EP014941BXQ
CSB-BP014941BXQ
CSB-MP014941BXQ
CSB-EP014941BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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