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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451ENT
CSB-EP015451ENT
CSB-BP015451ENT
CSB-MP015451ENT
CSB-EP015451ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DZY
CSB-EP009582DZY
CSB-BP009582DZY
CSB-MP009582DZY
CSB-EP009582DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Argininosuccinate synthase (argG) CSB-YP002234DZY
CSB-EP002234DZY
CSB-BP002234DZY
CSB-MP002234DZY
CSB-EP002234DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DZY
CSB-EP014799DZY
CSB-BP014799DZY
CSB-MP014799DZY
CSB-EP014799DZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus tRNA pseudouridine synthase A (truA) CSB-YP019086FOF
CSB-EP019086FOF
CSB-BP019086FOF
CSB-MP019086FOF
CSB-EP019086FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824FOF
CSB-EP005824FOF
CSB-BP005824FOF
CSB-MP005824FOF
CSB-EP005824FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus ATP synthase subunit alpha (atpA), partial CSB-YP002344FOF
CSB-EP002344FOF
CSB-BP002344FOF
CSB-MP002344FOF
CSB-EP002344FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Serine hydroxymethyltransferase (glyA) CSB-YP021274FOF
CSB-EP021274FOF
CSB-BP021274FOF
CSB-MP021274FOF
CSB-EP021274FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus Adenosylhomocysteinase (ahcY) CSB-YP001474FOF
CSB-EP001474FOF
CSB-BP001474FOF
CSB-MP001474FOF
CSB-EP001474FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces griseus subsp. griseus DNA ligase 1 (ligA1), partial CSB-YP012930FOF
CSB-EP012930FOF
CSB-BP012930FOF
CSB-MP012930FOF
CSB-EP012930FOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium urealyticum ATP synthase subunit delta (atpH) CSB-YP002355DXD
CSB-EP002355DXD
CSB-BP002355DXD
CSB-MP002355DXD
CSB-EP002355DXD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc citreum Translation initiation factor IF-2 (infB), partial CSB-YP015164LPD
CSB-EP015164LPD
CSB-BP015164LPD
CSB-MP015164LPD
CSB-EP015164LPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc citreum tRNA dimethylallyltransferase (miaA) CSB-YP024529LPD
CSB-EP024529LPD
CSB-BP024529LPD
CSB-MP024529LPD
CSB-EP024529LPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc citreum ATP synthase subunit beta (atpD) CSB-YP002350LPD
CSB-EP002350LPD
CSB-BP002350LPD
CSB-MP002350LPD
CSB-EP002350LPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leuconostoc citreum Elongation factor G (fusA), partial CSB-YP009373LPD
CSB-EP009373LPD
CSB-BP009373LPD
CSB-MP009373LPD
CSB-EP009373LPD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callicebus moloch Histone-lysine N-methyltransferase setd3 (SETD3), partial CSB-YP021104CYE
CSB-EP021104CYE
CSB-BP021104CYE
CSB-MP021104CYE
CSB-EP021104CYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium abscessus Elongation factor Ts (tsf) CSB-YP025124MVF
CSB-EP025124MVF
CSB-BP025124MVF
CSB-MP025124MVF
CSB-EP025124MVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium abscessus Triosephosphate isomerase (tpiA) CSB-YP024102MVF
CSB-EP024102MVF
CSB-BP024102MVF
CSB-MP024102MVF
CSB-EP024102MVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium radiotolerans tRNA pseudouridine synthase A (truA) CSB-YP019086MTD
CSB-EP019086MTD
CSB-BP019086MTD
CSB-MP019086MTD
CSB-EP019086MTD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium radiotolerans Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824MTD
CSB-EP005824MTD
CSB-BP005824MTD
CSB-MP005824MTD
CSB-EP005824MTD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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