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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermotoga sp. Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799TNM
CSB-EP014799TNM
CSB-BP014799TNM
CSB-MP014799TNM
CSB-EP014799TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. Elongation factor G (fusA), partial CSB-YP009373TNM
CSB-EP009373TNM
CSB-BP009373TNM
CSB-MP009373TNM
CSB-EP009373TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. ATP synthase subunit b (atpF), partial CSB-YP002358TNM1
CSB-EP002358TNM1
CSB-BP002358TNM1
CSB-MP002358TNM1
CSB-EP002358TNM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. 6-phosphofructokinase (pfkA) CSB-YP017822TNM
CSB-EP017822TNM
CSB-BP017822TNM
CSB-MP017822TNM
CSB-EP017822TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga sp. Translation initiation factor IF-2 (infB), partial CSB-YP015164TNM
CSB-EP015164TNM
CSB-BP015164TNM
CSB-MP015164TNM
CSB-EP015164TNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DUI
CSB-EP014799DUI
CSB-BP014799DUI
CSB-MP014799DUI
CSB-EP014799DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Peptide chain release factor 1 (prfA) CSB-YP015197DUI
CSB-EP015197DUI
CSB-BP015197DUI
CSB-MP015197DUI
CSB-EP015197DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Elongation factor Tu (tuf1) CSB-YP025342DUI
CSB-EP025342DUI
CSB-BP025342DUI
CSB-MP025342DUI
CSB-EP025342DUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Cysteine desulfurase (iscS) CSB-YP015769SYY
CSB-EP015769SYY
CSB-BP015769SYY
CSB-MP015769SYY
CSB-EP015769SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Transaldolase (tal) CSB-YP023112SYY
CSB-EP023112SYY
CSB-BP023112SYY
CSB-MP023112SYY
CSB-EP023112SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella woodyi Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493SYY
CSB-EP009493SYY
CSB-BP009493SYY
CSB-MP009493SYY
CSB-EP009493SYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Malate dehydrogenase (mdh) CSB-YP013623BXQ
CSB-EP013623BXQ
CSB-BP013623BXQ
CSB-MP013623BXQ
CSB-EP013623BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Translation initiation factor IF-2 (infB), partial CSB-YP015164BXQ
CSB-EP015164BXQ
CSB-BP015164BXQ
CSB-MP015164BXQ
CSB-EP015164BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493BXQ
CSB-EP009493BXQ
CSB-BP009493BXQ
CSB-MP009493BXQ
CSB-EP009493BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Galactokinase (galK) CSB-YP009198YAX
CSB-EP009198YAX
CSB-BP009198YAX
CSB-MP009198YAX
CSB-EP009198YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 GTPase Era (era) CSB-YP007759YAX
CSB-EP007759YAX
CSB-BP007759YAX
CSB-MP007759YAX
CSB-EP007759YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Transaldolase (tal) CSB-YP023112YAX
CSB-EP023112YAX
CSB-BP023112YAX
CSB-MP023112YAX
CSB-EP023112YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Cysteine desulfurase (sufS) CSB-YP015769YAX
CSB-EP015769YAX
CSB-BP015769YAX
CSB-MP015769YAX
CSB-EP015769YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Thymidylate kinase (tmk) CSB-YP007226YAX
CSB-EP007226YAX
CSB-BP007226YAX
CSB-MP007226YAX
CSB-EP007226YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP synthase subunit b (atpF), partial CSB-YP002358FGC1
CSB-EP002358FGC1
CSB-BP002358FGC1
CSB-MP002358FGC1
CSB-EP002358FGC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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