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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Uncharacterized protein ybjE (ybjE), partial CSB-YP301979ENV1
CSB-EP301979ENV1
CSB-BP301979ENV1
CSB-MP301979ENV1
CSB-EP301979ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0142 protein ycaO (ycaO) CSB-YP301980ENV
CSB-EP301980ENV
CSB-BP301980ENV
CSB-MP301980ENV
CSB-EP301980ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable fimbrial chaperone protein ElfD (elfD) CSB-YP301981ENV
CSB-EP301981ENV
CSB-BP301981ENV
CSB-MP301981ENV
CSB-EP301981ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein yccS (yccS), partial CSB-YP301982ENV
CSB-EP301982ENV
CSB-BP301982ENV
CSB-MP301982ENV
CSB-EP301982ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein gfcC (gfcC) CSB-YP301983ENV
CSB-EP301983ENV
CSB-BP301983ENV
CSB-MP301983ENV
CSB-EP301983ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable malonic semialdehyde reductase RutE (rutE) CSB-YP301984ENV
CSB-EP301984ENV
CSB-BP301984ENV
CSB-MP301984ENV
CSB-EP301984ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable diguanylate cyclase YcdT (ycdT), partial CSB-YP301985ENV
CSB-EP301985ENV
CSB-BP301985ENV
CSB-MP301985ENV
CSB-EP301985ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ycfL (ycfL) CSB-YP301986ENV
CSB-EP301986ENV
CSB-BP301986ENV
CSB-MP301986ENV
CSB-EP301986ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Prophage lambda integrase (intE) CSB-YP301987ENV
CSB-EP301987ENV
CSB-BP301987ENV
CSB-MP301987ENV
CSB-EP301987ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ymfM (ymfM) CSB-YP301988ENV
CSB-EP301988ENV
CSB-BP301988ENV
CSB-MP301988ENV
CSB-EP301988ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ycgX (ycgX) CSB-YP301989ENV
CSB-EP301989ENV
CSB-BP301989ENV
CSB-MP301989ENV
CSB-EP301989ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YcgG (ycgG) CSB-YP301990ENV
CSB-EP301990ENV
CSB-BP301990ENV
CSB-MP301990ENV
CSB-EP301990ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cell volume regulation protein A (cvrA), partial CSB-YP301991ENV
CSB-EP301991ENV
CSB-BP301991ENV
CSB-MP301991ENV
CSB-EP301991ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ychS (ychS) CSB-YP301992ENV
CSB-EP301992ENV
CSB-BP301992ENV
CSB-MP301992ENV
CSB-EP301992ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putrescine importer PuuP (puuP), partial CSB-YP301993ENV
CSB-EP301993ENV
CSB-BP301993ENV
CSB-MP301993ENV
CSB-EP301993ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP301994ENV
CSB-EP301994ENV
CSB-BP301994ENV
CSB-MP301994ENV
CSB-EP301994ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ydaW (ydaW) CSB-YP301995ENV
CSB-EP301995ENV
CSB-BP301995ENV
CSB-MP301995ENV
CSB-EP301995ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 1,2-phenylacetyl-CoA epoxidase, subunit A (paaA) CSB-YP301996ENV
CSB-EP301996ENV
CSB-BP301996ENV
CSB-MP301996ENV
CSB-EP301996ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acyl-coenzyme A thioesterase PaaI (paaI) CSB-YP301997ENV
CSB-EP301997ENV
CSB-BP301997ENV
CSB-MP301997ENV
CSB-EP301997ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Regulatory protein mokB (mokB) CSB-YP301998ENV
CSB-EP301998ENV
CSB-BP301998ENV
CSB-MP301998ENV
CSB-EP301998ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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