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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Zea mays Unknown protein from spot 474 of 2D-PAGE of etiolated coleoptile CSB-YP302182ZAX
CSB-EP302182ZAX
CSB-BP302182ZAX
CSB-MP302182ZAX
CSB-EP302182ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 907 of 2D-PAGE of etiolated coleoptile CSB-YP302183ZAX
CSB-EP302183ZAX
CSB-BP302183ZAX
CSB-MP302183ZAX
CSB-EP302183ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 67 of 2D-PAGE of etiolated coleoptile CSB-YP302184ZAX
CSB-EP302184ZAX
CSB-BP302184ZAX
CSB-MP302184ZAX
CSB-EP302184ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Unknown protein from spot 365 of 2D-PAGE of etiolated coleoptile CSB-YP302185ZAX
CSB-EP302185ZAX
CSB-BP302185ZAX
CSB-MP302185ZAX
CSB-EP302185ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua Chymotrypsin B CSB-YP302186GER
CSB-EP302186GER
CSB-BP302186GER
CSB-MP302186GER
CSB-EP302186GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Tetrahydromethanopterin S-methyltransferase subunit E (mtrE), partial CSB-YP302187MSN1
CSB-EP302187MSN1
CSB-BP302187MSN1
CSB-MP302187MSN1
CSB-EP302187MSN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina mazei Tetrahydromethanopterin S-methyltransferase subunit G (mtrG), partial CSB-YP302188MSN1
CSB-EP302188MSN1
CSB-BP302188MSN1
CSB-MP302188MSN1
CSB-EP302188MSN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Phosphoglycerate kinase, chloroplastic CSB-YP302189EVD
CSB-EP302189EVD
CSB-BP302189EVD
CSB-MP302189EVD
CSB-EP302189EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Oxygen-evolving enhancer protein 2 CSB-YP302190EVD
CSB-EP302190EVD
CSB-BP302190EVD
CSB-MP302190EVD
CSB-EP302190EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lantibiotic mutacin B-Ny266 CSB-YP302191FMR
CSB-EP302191FMR
CSB-BP302191FMR
CSB-MP302191FMR
CSB-EP302191FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Potassium channel toxin alpha-KTx 9.3 CSB-YP302192LDS
CSB-EP302192LDS
CSB-BP302192LDS
CSB-MP302192LDS
CSB-EP302192LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL) CSB-YP302193MVW
CSB-EP302193MVW
CSB-BP302193MVW
CSB-MP302193MVW
CSB-EP302193MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blaberus craniifer Cuticle protein 4 CSB-YP302194BHA
CSB-EP302194BHA
CSB-BP302194BHA
CSB-MP302194BHA
CSB-EP302194BHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Ferredoxin-thioredoxin reductase, variable chain CSB-YP302195ZAX
CSB-EP302195ZAX
CSB-BP302195ZAX
CSB-MP302195ZAX
CSB-EP302195ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tenebrio molitor Larval/pupal cuticle protein H1C (LPCP-22) CSB-YP302196TBF
CSB-EP302196TBF
CSB-BP302196TBF
CSB-MP302196TBF
CSB-EP302196TBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK) CSB-YP302197MVW
CSB-EP302197MVW
CSB-BP302197MVW
CSB-MP302197MVW
CSB-EP302197MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Trigger factor (tig) CSB-YP302198BRJ
CSB-EP302198BRJ
CSB-BP302198BRJ
CSB-MP302198BRJ
CSB-EP302198BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tuber borchii 11.9 kDa wall protein (TBF-1) CSB-YP302199TJE
CSB-EP302199TJE
CSB-BP302199TJE
CSB-MP302199TJE
CSB-EP302199TJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Styela clava Clavanin-D CSB-YP302200FOV
CSB-EP302200FOV
CSB-BP302200FOV
CSB-MP302200FOV
CSB-EP302200FOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Horse Carbohydrate-binding protein AWN CSB-YP302201HO
CSB-EP302201HO
CSB-BP302201HO
CSB-MP302201HO
CSB-EP302201HO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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