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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vaccinia virus Protein A30 (VACWR153) CSB-YP302384VAI
CSB-EP302384VAI
CSB-BP302384VAI
CSB-MP302384VAI
CSB-EP302384VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus Protein I2 (I2L), partial CSB-YP302385VAR1
CSB-EP302385VAR1
CSB-BP302385VAR1
CSB-MP302385VAR1
CSB-EP302385VAR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein A32 (VACWR155) CSB-YP302386VAI
CSB-EP302386VAI
CSB-BP302386VAI
CSB-MP302386VAI
CSB-EP302386VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein L5 (VACWR092), partial CSB-YP302387VAI1
CSB-EP302387VAI1
CSB-BP302387VAI1
CSB-MP302387VAI1
CSB-EP302387VAI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 8.9 kDa protein (A ORF T) CSB-YP302388VAA
CSB-EP302388VAA
CSB-BP302388VAA
CSB-MP302388VAA
CSB-EP302388VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin-like protein fixX (fixX) CSB-YP302390EGX
CSB-EP302390EGX
CSB-BP302390EGX
CSB-MP302390EGX
CSB-EP302390EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant P21 prophage-derived terminase small subunit (nohA) CSB-YP302391EGX
CSB-EP302391EGX
CSB-BP302391EGX
CSB-MP302391EGX
CSB-EP302391EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant P4 prophage-derived uncharacterized protein t2655 (t2655) CSB-YP302392SWW
CSB-EP302392SWW
CSB-BP302392SWW
CSB-MP302392SWW
CSB-EP302392SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phi-Lf prophage-derived putative minor coat protein (gIX-1) CSB-YP302393XAY
CSB-EP302393XAY
CSB-BP302393XAY
CSB-MP302393XAY
CSB-EP302393XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP302394EOD
CSB-EP302394EOD
CSB-BP302394EOD
CSB-MP302394EOD
CSB-EP302394EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthamoeba castellanii Profilin-1A CSB-YP302395AAJ
CSB-EP302395AAJ
CSB-BP302395AAJ
CSB-MP302395AAJ
CSB-EP302395AAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP302396EOD1
CSB-EP302396EOD1
CSB-BP302396EOD1
CSB-MP302396EOD1
CSB-EP302396EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA polymerase processivity factor component A20 (MVA132R, ACAM3000_MVA_132) CSB-YP302397VEE
CSB-EP302397VEE
CSB-BP302397VEE
CSB-MP302397VEE
CSB-EP302397VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus hebraeus Beta-insect excitatory toxin LqhIT1a CSB-YP302398LDS
CSB-EP302398LDS
CSB-BP302398LDS
CSB-MP302398LDS
CSB-EP302398LDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Acetylglutamate kinase (argB) CSB-YP302399BQB
CSB-EP302399BQB
CSB-BP302399BQB
CSB-MP302399BQB
CSB-EP302399BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Endonuclease V (nfi) CSB-YP302400ENV
CSB-EP302400ENV
CSB-BP302400ENV
CSB-MP302400ENV
CSB-EP302400ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA) CSB-YP302401IIX
CSB-EP302401IIX
CSB-BP302401IIX
CSB-MP302401IIX
CSB-EP302401IIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial CSB-YP302402IKB
CSB-EP302402IKB
CSB-BP302402IKB
CSB-MP302402IKB
CSB-EP302402IKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP302403SKX
CSB-EP302403SKX
CSB-BP302403SKX
CSB-MP302403SKX
CSB-EP302403SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Staphylokinase (sak) CSB-YP302404SKX
CSB-EP302404SKX
CSB-BP302404SKX
CSB-MP302404SKX
CSB-EP302404SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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