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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nicotiana tabacum ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP303140NHE1
CSB-EP303140NHE1
CSB-BP303140NHE1
CSB-MP303140NHE1
CSB-EP303140NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ferrous-iron efflux pump FieF (fieF), partial CSB-YP303141ENV1
CSB-EP303141ENV1
CSB-BP303141ENV1
CSB-MP303141ENV1
CSB-EP303141ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea rubens Photosystem I reaction center subunit IX (psaJ), partial CSB-YP303142EVN
CSB-EP303142EVN
CSB-BP303142EVN
CSB-MP303142EVN
CSB-EP303142EVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus thunbergii Cytochrome b6-f complex subunit 6 (petL), partial CSB-YP303143EVZ
CSB-EP303143EVZ
CSB-BP303143EVZ
CSB-MP303143EVZ
CSB-EP303143EVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Capsular synthesis regulator component B (rcsB) CSB-YP303144ENV
CSB-EP303144ENV
CSB-BP303144ENV
CSB-MP303144ENV
CSB-EP303144ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein rcsF (rcsF) CSB-YP303145ENV
CSB-EP303145ENV
CSB-BP303145ENV
CSB-MP303145ENV
CSB-EP303145ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acylphosphatase (acyP) CSB-YP303146MVZ
CSB-EP303146MVZ
CSB-BP303146MVZ
CSB-MP303146MVZ
CSB-EP303146MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sec-independent protein translocase protein TatB (tatB), partial CSB-YP303147EOD
CSB-EP303147EOD
CSB-BP303147EOD
CSB-MP303147EOD
CSB-EP303147EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Poly-beta-1,6-N-acetyl-D-glucosamine export protein (pgaA) CSB-YP303148ENV
CSB-EP303148ENV
CSB-BP303148ENV
CSB-MP303148ENV
CSB-EP303148ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate kinase (adk) CSB-YP303149EOD
CSB-EP303149EOD
CSB-BP303149EOD
CSB-MP303149EOD
CSB-EP303149EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta trigyna Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP303150BGL
CSB-EP303150BGL
CSB-BP303150BGL
CSB-MP303150BGL
CSB-EP303150BGL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenothera elata subsp. hookeri Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP303151OAS
CSB-EP303151OAS
CSB-BP303151OAS
CSB-MP303151OAS
CSB-EP303151OAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP303152CTX
CSB-EP303152CTX
CSB-BP303152CTX
CSB-MP303152CTX
CSB-EP303152CTX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco rattle virus 29 kDa protein CSB-YP303153TFL
CSB-EP303153TFL
CSB-BP303153TFL
CSB-MP303153TFL
CSB-EP303153TFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Phosphoprotein (P) CSB-YP303154RAH
CSB-BP303154RAH
CSB-MP303154RAH
CSB-EP303154RAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus imperialis Conotoxin Im11.4 CSB-YP303155DWA
CSB-EP303155DWA
CSB-BP303155DWA
CSB-MP303155DWA
CSB-EP303155DWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles gaucho Sphingomyelin phosphodiesterase D LgSicTox-alphaI-Loxn-A CSB-YP303156LJE
CSB-EP303156LJE
CSB-BP303156LJE
CSB-MP303156LJE
CSB-EP303156LJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pepper mild mottle virus Capsid protein (CP) CSB-YP303157PFJ
CSB-EP303157PFJ
CSB-BP303157PFJ
CSB-MP303157PFJ
CSB-EP303157PFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4) CSB-YP303158TGB
CSB-EP303158TGB
CSB-BP303158TGB
CSB-MP303158TGB
CSB-EP303158TGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Photosystem II reaction center protein M (psbM), partial CSB-YP303159EWE
CSB-EP303159EWE
CSB-BP303159EWE
CSB-MP303159EWE
CSB-EP303159EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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