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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), partial CSB-YP304397ENV1
CSB-EP304397ENV1
CSB-BP304397ENV1
CSB-MP304397ENV1
CSB-EP304397ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Esterase YdiI (ydiI) CSB-YP304398ENV
CSB-BP304398ENV
CSB-MP304398ENV
CSB-EP304398ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized ABC transporter ATP-binding protein YdcT (ydcT) CSB-YP304399ENV
CSB-EP304399ENV
CSB-BP304399ENV
CSB-MP304399ENV
CSB-EP304399ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans Glucose-6-phosphate 1-dehydrogenase (zwf) CSB-YP304400AYK
CSB-EP304400AYK
CSB-BP304400AYK
CSB-MP304400AYK
CSB-EP304400AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Xylose isomerase (xylA) CSB-YP304401BQU
CSB-EP304401BQU
CSB-BP304401BQU
CSB-MP304401BQU
CSB-EP304401BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Phosphate acetyltransferase (pta) CSB-YP304402DWY
CSB-EP304402DWY
CSB-BP304402DWY
CSB-MP304402DWY
CSB-EP304402DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein (nifH) CSB-YP304403HWA
CSB-EP304403HWA
CSB-BP304403HWA
CSB-MP304403HWA
CSB-EP304403HWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Dihydroorotase (pyrC) CSB-YP304404LMS
CSB-EP304404LMS
CSB-BP304404LMS
CSB-MP304404LMS
CSB-EP304404LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP304405MIA
CSB-EP304405MIA
CSB-BP304405MIA
CSB-MP304405MIA
CSB-EP304405MIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Bacterioferritin (bfr) CSB-YP304406FFZ
CSB-EP304406FFZ
CSB-BP304406FFZ
CSB-MP304406FFZ
CSB-EP304406FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase principal sigma factor hrdB (hrdB), partial CSB-YP304407SUW
CSB-EP304407SUW
CSB-BP304407SUW
CSB-MP304407SUW
CSB-EP304407SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Protein translocase subunit SecY (secY), partial CSB-YP304408SSQ
CSB-EP304408SSQ
CSB-BP304408SSQ
CSB-MP304408SSQ
CSB-EP304408SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein sll0944 (sll0944) CSB-YP304409SSQ
CSB-EP304409SSQ
CSB-BP304409SSQ
CSB-MP304409SSQ
CSB-EP304409SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine acetyltransferase (cysE) CSB-YP304410FLV
CSB-EP304410FLV
CSB-BP304410FLV
CSB-MP304410FLV
CSB-EP304410FLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA polymerase sigma-54 factor (rpoN) CSB-YP304411XAK
CSB-EP304411XAK
CSB-BP304411XAK
CSB-MP304411XAK
CSB-EP304411XAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae DNA topoisomerase 4 subunit B (parE), partial CSB-YP304412MLW
CSB-EP304412MLW
CSB-BP304412MLW
CSB-MP304412MLW
CSB-EP304412MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L20 (rplT) CSB-YP304413MLW
CSB-EP304413MLW
CSB-BP304413MLW
CSB-MP304413MLW
CSB-EP304413MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Pyruvate kinase (pyk), partial CSB-YP304414MLW
CSB-EP304414MLW
CSB-BP304414MLW
CSB-MP304414MLW
CSB-EP304414MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Fructose-6-phosphate aldolase 1 (fsaA) CSB-YP304415ENV
CSB-EP304415ENV
CSB-BP304415ENV
CSB-MP304415ENV
CSB-EP304415ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agaricus bisporus Histone H2B (htbA) CSB-YP304416AYJ
CSB-EP304416AYJ
CSB-BP304416AYJ
CSB-MP304416AYJ
CSB-EP304416AYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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