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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Synechocystis sp. Membrane-associated protein slr1513 (slr1513) CSB-YP305004SSQ
CSB-EP305004SSQ
CSB-BP305004SSQ
CSB-MP305004SSQ
CSB-EP305004SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 3-isopropylmalate dehydrogenase (leuB) CSB-YP305005SSQ
CSB-EP305005SSQ
CSB-BP305005SSQ
CSB-MP305005SSQ
CSB-EP305005SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized oxidoreductase sll0816 (sll0816) CSB-YP305006SSQ
CSB-EP305006SSQ
CSB-BP305006SSQ
CSB-MP305006SSQ
CSB-EP305006SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized transporter sll1263 (sll1263), partial CSB-YP305007SSQ1
CSB-EP305007SSQ1
CSB-BP305007SSQ1
CSB-MP305007SSQ1
CSB-EP305007SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein sll1895 (sll1895), partial CSB-YP305008SSQ
CSB-EP305008SSQ
CSB-BP305008SSQ
CSB-MP305008SSQ
CSB-EP305008SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP305009SSQ
CSB-EP305009SSQ
CSB-BP305009SSQ
CSB-MP305009SSQ
CSB-EP305009SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Inositol-1-monophosphatase (suhB) CSB-YP305010SSQ
CSB-EP305010SSQ
CSB-BP305010SSQ
CSB-MP305010SSQ
CSB-EP305010SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr1152 (slr1152) CSB-YP305011SSQ
CSB-EP305011SSQ
CSB-BP305011SSQ
CSB-MP305011SSQ
CSB-EP305011SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phosphoribosylamine--glycine ligase (purD) CSB-YP305012SSQ
CSB-EP305012SSQ
CSB-BP305012SSQ
CSB-MP305012SSQ
CSB-EP305012SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. D-alanyl-D-alanine dipeptidase (ddpX) CSB-YP305013SSQ
CSB-EP305013SSQ
CSB-BP305013SSQ
CSB-MP305013SSQ
CSB-EP305013SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP305014SSQ
CSB-EP305014SSQ
CSB-BP305014SSQ
CSB-MP305014SSQ
CSB-EP305014SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP305015SSQ
CSB-EP305015SSQ
CSB-BP305015SSQ
CSB-MP305015SSQ
CSB-EP305015SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Maf-like protein sll0905 (sll0905) CSB-YP305016SSQ
CSB-EP305016SSQ
CSB-BP305016SSQ
CSB-MP305016SSQ
CSB-EP305016SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. SsrA-binding protein (smpB) CSB-YP305017SSQ
CSB-EP305017SSQ
CSB-BP305017SSQ
CSB-MP305017SSQ
CSB-EP305017SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative diflavin flavoprotein A 3 (dfa3), partial CSB-YP305018SSQ
CSB-EP305018SSQ
CSB-BP305018SSQ
CSB-MP305018SSQ
CSB-EP305018SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Alanine--tRNA ligase (alaS), partial CSB-YP305020SSQ
CSB-EP305020SSQ
CSB-BP305020SSQ
CSB-MP305020SSQ
CSB-EP305020SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uridylate kinase (pyrH) CSB-YP305021SSQ
CSB-EP305021SSQ
CSB-BP305021SSQ
CSB-MP305021SSQ
CSB-EP305021SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. KaiC-like protein 2 (slr1942), partial CSB-YP305022SSQ
CSB-EP305022SSQ
CSB-BP305022SSQ
CSB-MP305022SSQ
CSB-EP305022SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Octanoyltransferase (lipB) CSB-YP305023SSQ
CSB-EP305023SSQ
CSB-BP305023SSQ
CSB-MP305023SSQ
CSB-EP305023SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP305024SSQ
CSB-EP305024SSQ
CSB-BP305024SSQ
CSB-MP305024SSQ
CSB-EP305024SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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