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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Uncharacterized protein yfeY (yfeY) CSB-YP304357ENV
CSB-EP304357ENV
CSB-BP304357ENV
CSB-MP304357ENV
CSB-EP304357ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yffM (yffM) CSB-YP304358ENV
CSB-EP304358ENV
CSB-BP304358ENV
CSB-MP304358ENV
CSB-EP304358ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ethanolamine utilization protein eutA (eutA) CSB-YP304359ENV
CSB-EP304359ENV
CSB-BP304359ENV
CSB-MP304359ENV
CSB-EP304359ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Esterase YpfH (ypfH) CSB-YP304360ENV
CSB-EP304360ENV
CSB-BP304360ENV
CSB-MP304360ENV
CSB-EP304360ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Penicillin-binding protein 1C (pbpC), partial CSB-YP304361ENV
CSB-EP304361ENV
CSB-BP304361ENV
CSB-MP304361ENV
CSB-EP304361ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ypjC (ypjC) CSB-YP304362ENV
CSB-EP304362ENV
CSB-BP304362ENV
CSB-MP304362ENV
CSB-EP304362ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ygeH (ygeH) CSB-YP304363ENV
CSB-EP304363ENV
CSB-BP304363ENV
CSB-MP304363ENV
CSB-EP304363ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Porin ompL (ompL) CSB-YP304364ENV
CSB-EP304364ENV
CSB-BP304364ENV
CSB-MP304364ENV
CSB-EP304364ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein hokE (hokE), partial CSB-YP304365ENV
CSB-EP304365ENV
CSB-BP304365ENV
CSB-MP304365ENV
CSB-EP304365ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane ABC transporter permease protein ydcU (ydcU), partial CSB-YP304366ENV
CSB-EP304366ENV
CSB-BP304366ENV
CSB-MP304366ENV
CSB-EP304366ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ydcI (ydcI) CSB-YP304367ENV
CSB-EP304367ENV
CSB-BP304367ENV
CSB-MP304367ENV
CSB-EP304367ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) CSB-YP304368ENV
CSB-EP304368ENV
CSB-BP304368ENV
CSB-MP304368ENV
CSB-EP304368ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative outer membrane usher protein yfcU (yfcU), partial CSB-YP304369ENV
CSB-EP304369ENV
CSB-BP304369ENV
CSB-MP304369ENV
CSB-EP304369ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cation efflux system protein CusF (cusF) CSB-YP304370ENV
CSB-EP304370ENV
CSB-BP304370ENV
CSB-MP304370ENV
CSB-EP304370ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybeQ (ybeQ) CSB-YP304371ENV
CSB-EP304371ENV
CSB-BP304371ENV
CSB-MP304371ENV
CSB-EP304371ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized oxidoreductase YdjG (ydjG) CSB-YP304372ENV
CSB-EP304372ENV
CSB-BP304372ENV
CSB-MP304372ENV
CSB-EP304372ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ABC transporter periplasmic-binding protein yphF (yphF) CSB-YP304373ENV
CSB-EP304373ENV
CSB-BP304373ENV
CSB-MP304373ENV
CSB-EP304373ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized fimbrial-like protein yfcV (yfcV) CSB-YP304374ENV
CSB-EP304374ENV
CSB-BP304374ENV
CSB-MP304374ENV
CSB-EP304374ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable diguanylate cyclase YdaM (ydaM) CSB-YP304375ENV
CSB-EP304375ENV
CSB-BP304375ENV
CSB-MP304375ENV
CSB-EP304375ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator yneJ (yneJ) CSB-YP304376ENV
CSB-EP304376ENV
CSB-BP304376ENV
CSB-MP304376ENV
CSB-EP304376ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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