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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_281 (MPN_281) CSB-YP304278MLW
CSB-EP304278MLW
CSB-BP304278MLW
CSB-MP304278MLW
CSB-EP304278MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG134 homolog (MPN_275) CSB-YP304279MLW
CSB-EP304279MLW
CSB-BP304279MLW
CSB-MP304279MLW
CSB-EP304279MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP304280MLW
CSB-EP304280MLW
CSB-BP304280MLW
CSB-MP304280MLW
CSB-EP304280MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG121 homolog (MPN_260), partial CSB-YP304281MLW
CSB-EP304281MLW
CSB-BP304281MLW
CSB-MP304281MLW
CSB-EP304281MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Asparagine--tRNA ligase (asnS) CSB-YP304282MLW
CSB-EP304282MLW
CSB-BP304282MLW
CSB-MP304282MLW
CSB-EP304282MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG105 homolog (MPN_244), partial CSB-YP304283MLW
CSB-EP304283MLW
CSB-BP304283MLW
CSB-MP304283MLW
CSB-EP304283MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MG095 homolog (MPN_233) CSB-YP304284MLW
CSB-EP304284MLW
CSB-BP304284MLW
CSB-MP304284MLW
CSB-EP304284MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Oligopeptide transport system permease protein oppC (oppC), partial CSB-YP304285MLW1
CSB-EP304285MLW1
CSB-BP304285MLW1
CSB-MP304285MLW1
CSB-EP304285MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized adenine-specific methylase MPN_108 (MPN_108) CSB-YP304286MLW
CSB-EP304286MLW
CSB-BP304286MLW
CSB-MP304286MLW
CSB-EP304286MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_101 (MPN_101) CSB-YP304287MLW
CSB-EP304287MLW
CSB-BP304287MLW
CSB-MP304287MLW
CSB-EP304287MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 30S ribosomal protein S19 (rpsS) CSB-YP304288MLW
CSB-EP304288MLW
CSB-BP304288MLW
CSB-MP304288MLW
CSB-EP304288MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG144 homolog (MPN_157), partial CSB-YP304289MLW1
CSB-EP304289MLW1
CSB-BP304289MLW1
CSB-MP304289MLW1
CSB-EP304289MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative adhesin P1-like protein MPN_099 (MPN_099) CSB-YP304290MLW
CSB-EP304290MLW
CSB-BP304290MLW
CSB-MP304290MLW
CSB-EP304290MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_091 (MPN_091) CSB-YP304291MLW
CSB-EP304291MLW
CSB-BP304291MLW
CSB-MP304291MLW
CSB-EP304291MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_086 (MPN_086), partial CSB-YP304292MLW1
CSB-EP304292MLW1
CSB-BP304292MLW1
CSB-MP304292MLW1
CSB-EP304292MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0174 protein yaaW (yaaW) CSB-YP304293ENV
CSB-EP304293ENV
CSB-BP304293ENV
CSB-MP304293ENV
CSB-EP304293ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insN for insertion sequence element IS911A (insN1) CSB-YP304294ENV
CSB-EP304294ENV
CSB-BP304294ENV
CSB-MP304294ENV
CSB-EP304294ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YkgI (ykgI) CSB-YP304295ENV
CSB-EP304295ENV
CSB-BP304295ENV
CSB-MP304295ENV
CSB-EP304295ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybbV (ybbV) CSB-YP304296ENV
CSB-EP304296ENV
CSB-BP304296ENV
CSB-MP304296ENV
CSB-EP304296ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Citrate lyase alpha chain (citF) CSB-YP304297ENV
CSB-EP304297ENV
CSB-BP304297ENV
CSB-MP304297ENV
CSB-EP304297ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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