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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Anemonia erythraea Toxin AETX-1 CSB-YP304880AKD
CSB-EP304880AKD
CSB-BP304880AKD
CSB-MP304880AKD
CSB-EP304880AKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enolase (eno) CSB-YP304881SMU
CSB-EP304881SMU
CSB-BP304881SMU
CSB-MP304881SMU
CSB-EP304881SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low calcium response locus protein R (lcrR) CSB-YP304882YAS
CSB-EP304882YAS
CSB-BP304882YAS
CSB-MP304882YAS
CSB-EP304882YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein psaB (psaB) CSB-YP304883YAH
CSB-EP304883YAH
CSB-BP304883YAH
CSB-MP304883YAH
CSB-EP304883YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation lipoprotein J (yscJ), partial CSB-YP304884YAS1
CSB-EP304884YAS1
CSB-BP304884YAS1
CSB-MP304884YAS1
CSB-EP304884YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein R (yscR), partial CSB-YP304885YAS1
CSB-EP304885YAS1
CSB-BP304885YAS1
CSB-MP304885YAS1
CSB-EP304885YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein U (yscU), partial CSB-YP304886YAH1
CSB-EP304886YAH1
CSB-BP304886YAH1
CSB-MP304886YAH1
CSB-EP304886YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease subunit gamma (ureA) CSB-YP304887YAH
CSB-EP304887YAH
CSB-BP304887YAH
CSB-MP304887YAH
CSB-EP304887YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Steroidogenic acute regulatory protein, mitochondrial (STAR) CSB-YP304888MRG
CSB-EP304888MRG
CSB-BP304888MRG
CSB-MP304888MRG
CSB-EP304888MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Friend virus susceptibility protein 1 (Fv1) CSB-YP304889MO
CSB-EP304889MO
CSB-BP304889MO
CSB-MP304889MO
CSB-EP304889MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Homeobox protein TGIF1 (Tgif1) CSB-YP304890MO
CSB-EP304890MO
CSB-BP304890MO
CSB-MP304890MO
CSB-EP304890MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Testis-specific protein TSX (Tsx) CSB-YP304891RA
CSB-EP304891RA
CSB-BP304891RA
CSB-MP304891RA
CSB-EP304891RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP304894DPS
CSB-EP304894DPS
CSB-BP304894DPS
CSB-MP304894DPS
CSB-EP304894DPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosome initiation inhibitor (iciA) CSB-YP304895AXZ
CSB-EP304895AXZ
CSB-BP304895AXZ
CSB-MP304895AXZ
CSB-EP304895AXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium vitis Putative tartrate transporter (ttuB), partial CSB-YP304896AYT
CSB-EP304896AYT
CSB-BP304896AYT
CSB-MP304896AYT
CSB-EP304896AYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Riboflavin biosynthesis protein RibD (ribD) CSB-YP304898BQB
CSB-EP304898BQB
CSB-BP304898BQB
CSB-MP304898BQB
CSB-EP304898BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Uncharacterized membrane protein BB_D15 (BB_D15), partial CSB-YP304899BUD1
CSB-EP304899BUD1
CSB-BP304899BUD1
CSB-MP304899BUD1
CSB-EP304899BUD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine--tRNA ligase (alaS), partial CSB-YP304900BSE
CSB-EP304900BSE
CSB-BP304900BSE
CSB-MP304900BSE
CSB-EP304900BSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis Tetracycline resistance protein tetQ (tetQ), partial CSB-YP304901BDQ
CSB-EP304901BDQ
CSB-BP304901BDQ
CSB-MP304901BDQ
CSB-EP304901BDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 15/16 (vlp15) CSB-YP304902BIP
CSB-EP304902BIP
CSB-BP304902BIP
CSB-MP304902BIP
CSB-EP304902BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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