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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG280 homolog (MPN_399), partial CSB-YP305086MLW1
CSB-EP305086MLW1
CSB-BP305086MLW1
CSB-MP305086MLW1
CSB-EP305086MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG267 homolog (MPN_385), partial CSB-YP305087MLW1
CSB-EP305087MLW1
CSB-BP305087MLW1
CSB-MP305087MLW1
CSB-EP305087MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae DNA polymerase III subunit alpha (dnaE), partial CSB-YP305088MLW
CSB-EP305088MLW
CSB-BP305088MLW
CSB-MP305088MLW
CSB-EP305088MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_369 (MPN_369) CSB-YP305089MLW
CSB-EP305089MLW
CSB-BP305089MLW
CSB-MP305089MLW
CSB-EP305089MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative tRNA (adenine-N (1)-)-methyltransferase MPN_351 CSB-YP305090MLW
CSB-EP305090MLW
CSB-BP305090MLW
CSB-MP305090MLW
CSB-EP305090MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative type-1 restriction enzyme MpnORFDP R protein part 2 (MPN_345) CSB-YP305091MLW
CSB-EP305091MLW
CSB-BP305091MLW
CSB-MP305091MLW
CSB-EP305091MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG241 homolog (MPN_337), partial CSB-YP305092MLW1
CSB-EP305092MLW1
CSB-BP305092MLW1
CSB-MP305092MLW1
CSB-EP305092MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L35 (rpmI) CSB-YP305093MLW
CSB-EP305093MLW
CSB-BP305093MLW
CSB-MP305093MLW
CSB-EP305093MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG237 homolog (MPN_330) CSB-YP305094MLW
CSB-EP305094MLW
CSB-BP305094MLW
CSB-MP305094MLW
CSB-EP305094MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG226 homolog (MPN_319), partial CSB-YP305095MLW
CSB-EP305095MLW
CSB-BP305095MLW
CSB-MP305095MLW
CSB-EP305095MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG218.1 homolog (MPN_311) CSB-YP305096MLW
CSB-BP305096MLW
CSB-MP305096MLW
CSB-EP305096MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Segregation and condensation protein A (scpA), partial CSB-YP305097MLW
CSB-EP305097MLW
CSB-BP305097MLW
CSB-MP305097MLW
CSB-EP305097MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Lipoprotein signal peptidase (lspA), partial CSB-YP305098MLW1
CSB-EP305098MLW1
CSB-BP305098MLW1
CSB-MP305098MLW1
CSB-EP305098MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_284 (MPN_284), partial CSB-YP305099MLW
CSB-EP305099MLW
CSB-BP305099MLW
CSB-MP305099MLW
CSB-EP305099MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG135 homolog (MPN_276), partial CSB-YP305100MLW1
CSB-EP305100MLW1
CSB-BP305100MLW1
CSB-MP305100MLW1
CSB-EP305100MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG127 homolog (MPN_266) CSB-YP305101MLW
CSB-EP305101MLW
CSB-BP305101MLW
CSB-MP305101MLW
CSB-EP305101MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG123 homolog (MPN_262), partial CSB-YP305102MLW1
CSB-EP305102MLW1
CSB-BP305102MLW1
CSB-MP305102MLW1
CSB-EP305102MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 30S ribosomal protein S7 (rpsG) CSB-YP305103MLW
CSB-EP305103MLW
CSB-BP305103MLW
CSB-MP305103MLW
CSB-EP305103MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Oligopeptide transport ATP-binding protein OppF (oppF), partial CSB-YP305104MLW
CSB-EP305104MLW
CSB-BP305104MLW
CSB-MP305104MLW
CSB-EP305104MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG075 homolog (MPN_213), partial CSB-YP305105MLW
CSB-EP305105MLW
CSB-BP305105MLW
CSB-MP305105MLW
CSB-EP305105MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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