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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Uncharacterized fimbrial chaperone yfcS (yfcS) CSB-YP305207ENV
CSB-EP305207ENV
CSB-BP305207ENV
CSB-MP305207ENV
CSB-EP305207ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-asparagine permease (ansP), partial CSB-YP305208ENV
CSB-EP305208ENV
CSB-BP305208ENV
CSB-MP305208ENV
CSB-EP305208ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Carbamate kinase-like protein YahI (yahI) CSB-YP305209ENV
CSB-EP305209ENV
CSB-BP305209ENV
CSB-MP305209ENV
CSB-EP305209ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ynaA (ynaA) CSB-YP305210ENV
CSB-EP305210ENV
CSB-BP305210ENV
CSB-MP305210ENV
CSB-EP305210ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Bactoprenol-linked glucose translocase homolog from prophage CPS-53 (yfdG), partial CSB-YP305211ENV1
CSB-EP305211ENV1
CSB-BP305211ENV1
CSB-MP305211ENV1
CSB-EP305211ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yagW (yagW) CSB-YP305212ENV
CSB-EP305212ENV
CSB-BP305212ENV
CSB-MP305212ENV
CSB-EP305212ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator yahB (yahB) CSB-YP305213ENV
CSB-EP305213ENV
CSB-BP305213ENV
CSB-MP305213ENV
CSB-EP305213ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator RhmR (rhmR) CSB-YP305214ENV
CSB-EP305214ENV
CSB-BP305214ENV
CSB-MP305214ENV
CSB-EP305214ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli HTH-type transcriptional regulator AbgR (abgR) CSB-YP305215ENV
CSB-EP305215ENV
CSB-BP305215ENV
CSB-MP305215ENV
CSB-EP305215ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 7-cyano-7-deazaguanine synthase (queC) CSB-YP305216ENV
CSB-EP305216ENV
CSB-BP305216ENV
CSB-MP305216ENV
CSB-EP305216ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Epimerase family protein yfcH (yfcH) CSB-YP305217ENV
CSB-EP305217ENV
CSB-BP305217ENV
CSB-MP305217ENV
CSB-EP305217ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-alanyl-D-alanine dipeptidase (ddpX) CSB-YP305218ENV
CSB-EP305218ENV
CSB-BP305218ENV
CSB-MP305218ENV
CSB-EP305218ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsZ (ftsZ) CSB-YP305219DPX
CSB-EP305219DPX
CSB-BP305219DPX
CSB-MP305219DPX
CSB-EP305219DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Urease subunit alpha (ureC), partial CSB-YP305220BRJ
CSB-EP305220BRJ
CSB-BP305220BRJ
CSB-MP305220BRJ
CSB-EP305220BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein beta chain (nifK) CSB-YP305221HWA
CSB-EP305221HWA
CSB-BP305221HWA
CSB-MP305221HWA
CSB-EP305221HWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), partial CSB-YP305222LMS
CSB-EP305222LMS
CSB-BP305222LMS
CSB-MP305222LMS
CSB-EP305222LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP305223MVZ
CSB-EP305223MVZ
CSB-BP305223MVZ
CSB-MP305223MVZ
CSB-EP305223MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi Aspartate carbamoyltransferase regulatory chain (pyrI) CSB-YP305224FHV
CSB-EP305224FHV
CSB-BP305224FHV
CSB-MP305224FHV
CSB-EP305224FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-glutamine:scyllo-inosose aminotransferase (stsC) CSB-YP305225SUW
CSB-EP305225SUW
CSB-BP305225SUW
CSB-MP305225SUW
CSB-EP305225SUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP305226SSQ
CSB-EP305226SSQ
CSB-BP305226SSQ
CSB-MP305226SSQ
CSB-EP305226SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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