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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Mycoplasma pneumoniae Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP305106MLW
CSB-EP305106MLW
CSB-BP305106MLW
CSB-MP305106MLW
CSB-EP305106MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_206 (MPN_206) CSB-YP305107MLW
CSB-EP305107MLW
CSB-BP305107MLW
CSB-MP305107MLW
CSB-EP305107MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L4 (rplD) CSB-YP305108MLW
CSB-EP305108MLW
CSB-BP305108MLW
CSB-MP305108MLW
CSB-EP305108MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UPF0053 protein MG146 homolog (MPN_159), partial CSB-YP305109MLW1
CSB-EP305109MLW1
CSB-BP305109MLW1
CSB-MP305109MLW1
CSB-EP305109MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_098 (MPN_098) CSB-YP305110MLW
CSB-EP305110MLW
CSB-BP305110MLW
CSB-MP305110MLW
CSB-EP305110MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_090 (MPN_090), partial CSB-YP305111MLW1
CSB-EP305111MLW1
CSB-BP305111MLW1
CSB-MP305111MLW1
CSB-EP305111MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_084 (MPN_084), partial CSB-YP305112MLW
CSB-EP305112MLW
CSB-BP305112MLW
CSB-MP305112MLW
CSB-EP305112MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yaaX (yaaX) CSB-YP305113ENV
CSB-EP305113ENV
CSB-BP305113ENV
CSB-MP305113ENV
CSB-EP305113ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ykfA (ykfA) CSB-YP305114ENV
CSB-EP305114ENV
CSB-BP305114ENV
CSB-MP305114ENV
CSB-EP305114ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein ykgB (ykgB), partial CSB-YP305115ENV1
CSB-EP305115ENV1
CSB-BP305115ENV1
CSB-MP305115ENV1
CSB-EP305115ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ykiA (ykiA) CSB-YP305116ENV
CSB-EP305116ENV
CSB-BP305116ENV
CSB-MP305116ENV
CSB-EP305116ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ybCC (ybcC) CSB-YP305117ENV
CSB-EP305117ENV
CSB-BP305117ENV
CSB-MP305117ENV
CSB-EP305117ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Esterase ybfF (ybfF) CSB-YP305118ENV
CSB-EP305118ENV
CSB-BP305118ENV
CSB-MP305118ENV
CSB-EP305118ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybgO (ybgO) CSB-YP305119ENV
CSB-EP305119ENV
CSB-BP305119ENV
CSB-MP305119ENV
CSB-EP305119ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YbhJ (ybhJ) CSB-YP305120ENV
CSB-EP305120ENV
CSB-BP305120ENV
CSB-MP305120ENV
CSB-EP305120ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP305121ENV
CSB-EP305121ENV
CSB-BP305121ENV
CSB-MP305121ENV
CSB-EP305121ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutathione import ATP-binding protein GsiA (gsiA) CSB-YP305122ENV
CSB-EP305122ENV
CSB-BP305122ENV
CSB-MP305122ENV
CSB-EP305122ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative lipoprotein YliF (yliF), partial CSB-YP305123ENV1
CSB-EP305123ENV1
CSB-BP305123ENV1
CSB-MP305123ENV1
CSB-EP305123ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized lipoprotein ybjP (ybjP) CSB-YP305124ENV
CSB-EP305124ENV
CSB-BP305124ENV
CSB-MP305124ENV
CSB-EP305124ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybjD (ybjD) CSB-YP305125ENV
CSB-EP305125ENV
CSB-BP305125ENV
CSB-MP305125ENV
CSB-EP305125ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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