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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP309802DXP
CSB-EP309802DXP
CSB-BP309802DXP
CSB-MP309802DXP
CSB-EP309802DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium perfringens Urease subunit beta (ureB) CSB-YP309803CMB
CSB-EP309803CMB
CSB-BP309803CMB
CSB-MP309803CMB
CSB-EP309803CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase, dissimilatory-type subunit beta (dsrB) CSB-YP309804DJO
CSB-EP309804DJO
CSB-BP309804DJO
CSB-MP309804DJO
CSB-EP309804DJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica 10 kDa chaperonin (groS) CSB-YP309805FCO
CSB-EP309805FCO
CSB-BP309805FCO
CSB-MP309805FCO
CSB-EP309805FCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Gamma-glutamyl phosphate reductase (proA) CSB-YP309806LOS
CSB-EP309806LOS
CSB-BP309806LOS
CSB-MP309806LOS
CSB-EP309806LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanopyrus kandleri Methenyltetrahydromethanopterin cyclohydrolase (mch) CSB-YP309807MSI
CSB-EP309807MSI
CSB-BP309807MSI
CSB-MP309807MSI
CSB-EP309807MSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin VapB20 (vapB20) CSB-YP309808MVZ
CSB-EP309808MVZ
CSB-BP309808MVZ
CSB-MP309808MVZ
CSB-EP309808MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB18 (vapB18) CSB-YP309809MVZ
CSB-EP309809MVZ
CSB-BP309809MVZ
CSB-MP309809MVZ
CSB-EP309809MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative mycofactocin system protein MftB (mftB) CSB-YP309810MVZ
CSB-EP309810MVZ
CSB-BP309810MVZ
CSB-MP309810MVZ
CSB-EP309810MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L29 (rpmC) CSB-YP309811MVZ
CSB-EP309811MVZ
CSB-BP309811MVZ
CSB-MP309811MVZ
CSB-EP309811MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-binding protein HU homolog (hup) CSB-YP309812MVZ
CSB-EP309812MVZ
CSB-BP309812MVZ
CSB-MP309812MVZ
CSB-EP309812MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trans-acting enoyl reductase (Rv2953, MT3027) CSB-YP309813MVZ
CSB-EP309813MVZ
CSB-BP309813MVZ
CSB-MP309813MVZ
CSB-EP309813MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP309814MVZ
CSB-EP309814MVZ
CSB-BP309814MVZ
CSB-MP309814MVZ
CSB-EP309814MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulator BlaI (blaI) CSB-YP309815MVZ
CSB-EP309815MVZ
CSB-BP309815MVZ
CSB-MP309815MVZ
CSB-EP309815MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine acetyltransferase (cysE) CSB-YP309816MVZ
CSB-EP309816MVZ
CSB-BP309816MVZ
CSB-MP309816MVZ
CSB-EP309816MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospholipase C 2 (plcB) CSB-YP309817MVZ
CSB-EP309817MVZ
CSB-BP309817MVZ
CSB-MP309817MVZ
CSB-EP309817MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SecB-like chaperone Rv1957 (secBL) CSB-YP309818MVZ
CSB-EP309818MVZ
CSB-BP309818MVZ
CSB-MP309818MVZ
CSB-EP309818MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv1945/MT1995 (Rv1945, MT1995) CSB-YP309819MVZ
CSB-EP309819MVZ
CSB-BP309819MVZ
CSB-MP309819MVZ
CSB-EP309819MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP309820MVZ
CSB-EP309820MVZ
CSB-BP309820MVZ
CSB-MP309820MVZ
CSB-EP309820MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Flagellin B3 (flaB3) CSB-YP309821MNS
CSB-EP309821MNS
CSB-BP309821MNS
CSB-MP309821MNS
CSB-EP309821MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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