Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Proline iminopeptidase (PIP) CSB-YP309394DOA
CSB-EP309394DOA
CSB-BP309394DOA
CSB-MP309394DOA
CSB-EP309394DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Protein MLO (MLO), partial CSB-YP309395HWQ1
CSB-EP309395HWQ1
CSB-BP309395HWQ1
CSB-MP309395HWQ1
CSB-EP309395HWQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL1 (ATL1), partial CSB-YP309396DOA1
CSB-EP309396DOA1
CSB-BP309396DOA1
CSB-MP309396DOA1
CSB-EP309396DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Liriope muscari Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP309397LGS
CSB-EP309397LGS
CSB-BP309397LGS
CSB-MP309397LGS
CSB-EP309397LGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP309398PTE
CSB-EP309398PTE
CSB-BP309398PTE
CSB-MP309398PTE
CSB-EP309398PTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP309399SEJ
CSB-EP309399SEJ
CSB-BP309399SEJ
CSB-MP309399SEJ
CSB-EP309399SEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acacia farnesiana Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP309400AAH
CSB-EP309400AAH
CSB-BP309400AAH
CSB-MP309400AAH
CSB-EP309400AAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Germin-like protein subfamily 3 member 1 (GLP1) CSB-YP309401DOA
CSB-EP309401DOA
CSB-BP309401DOA
CSB-MP309401DOA
CSB-EP309401DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus pinatubonensis Carboxymethylenebutenolidase 2 (tfdEII) CSB-YP309402DZR
CSB-EP309402DZR
CSB-BP309402DZR
CSB-MP309402DZR
CSB-EP309402DZR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mercuric transport protein periplasmic component (merP) CSB-YP309403AGP
CSB-EP309403AGP
CSB-BP309403AGP
CSB-MP309403AGP
CSB-EP309403AGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas salmonicida Protein RecA (recA) CSB-YP309404AUI
CSB-EP309404AUI
CSB-BP309404AUI
CSB-MP309404AUI
CSB-EP309404AUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi 30S ribosomal protein S10 (rpsJ) CSB-YP309405BUD
CSB-EP309405BUD
CSB-BP309405BUD
CSB-MP309405BUD
CSB-EP309405BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Chaperone protein DnaJ (dnaJ) CSB-YP309406BVW
CSB-EP309406BVW
CSB-BP309406BVW
CSB-MP309406BVW
CSB-EP309406BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Indole-3-glycerol phosphate synthase (trpC) CSB-YP309407BVW
CSB-EP309407BVW
CSB-BP309407BVW
CSB-MP309407BVW
CSB-EP309407BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Maltodextrin import ATP-binding protein MsmX (msmX) CSB-YP309408BRJ
CSB-EP309408BRJ
CSB-BP309408BRJ
CSB-MP309408BRJ
CSB-EP309408BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cytochrome d ubiquinol oxidase subunit 2 (cydB), partial CSB-YP309409BRJ
CSB-EP309409BRJ
CSB-BP309409BRJ
CSB-MP309409BRJ
CSB-EP309409BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Negative regulatory protein yxlD (yxlD), partial CSB-YP309410BRJ1
CSB-EP309410BRJ1
CSB-BP309410BRJ1
CSB-MP309410BRJ1
CSB-EP309410BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ycgK (ycgK) CSB-YP309411BRJ
CSB-EP309411BRJ
CSB-BP309411BRJ
CSB-MP309411BRJ
CSB-EP309411BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phenolic acid decarboxylase subunit B (bsdB) CSB-YP309412BRJ
CSB-EP309412BRJ
CSB-BP309412BRJ
CSB-MP309412BRJ
CSB-EP309412BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter permease yclI (yclI), partial CSB-YP309413BRJ1
CSB-EP309413BRJ1
CSB-BP309413BRJ1
CSB-MP309413BRJ1
CSB-EP309413BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>