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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00370 (AtMg00370), partial CSB-YP308626DOA1
CSB-EP308626DOA1
CSB-BP308626DOA1
CSB-MP308626DOA1
CSB-EP308626DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00450 (AtMg00450) CSB-YP308627DOA
CSB-EP308627DOA
CSB-BP308627DOA
CSB-MP308627DOA
CSB-EP308627DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00660 (AtMg00660) CSB-YP308628DOA
CSB-EP308628DOA
CSB-BP308628DOA
CSB-MP308628DOA
CSB-EP308628DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago truncatula Early nodulin-20 (ENOD20) CSB-YP308629MQP
CSB-EP308629MQP
CSB-BP308629MQP
CSB-MP308629MQP
CSB-EP308629MQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Actin-53 CSB-YP308630NHE
CSB-EP308630NHE
CSB-BP308630NHE
CSB-MP308630NHE
CSB-EP308630NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-C1-1 (Os09g0504400, LOC_Os09g32680) CSB-YP308631OFG
CSB-EP308631OFG
CSB-BP308631OFG
CSB-MP308631OFG
CSB-EP308631OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Delta (24)-sterol reductase, partial CSB-YP308632EWE
CSB-EP308632EWE
CSB-BP308632EWE
CSB-MP308632EWE
CSB-EP308632EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Lon protease homolog 2, peroxisomal (LON1), partial CSB-YP308633ZAX
CSB-EP308633ZAX
CSB-BP308633ZAX
CSB-MP308633ZAX
CSB-EP308633ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Lon protease homolog 1, mitochondrial (LON1), partial CSB-YP308634DOA
CSB-EP308634DOA
CSB-BP308634DOA
CSB-MP308634DOA
CSB-EP308634DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Serpin-Z1B CSB-YP308635TQN
CSB-BP308635TQN
CSB-MP308635TQN
CSB-EP308635TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsicum annuum Acid beta-fructofuranosidase AIV-18, partial CSB-YP308636DQD1
CSB-EP308636DQD1
CSB-BP308636DQD1
CSB-MP308636DQD1
CSB-EP308636DQD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Remorin CSB-YP308637FIG
CSB-EP308637FIG
CSB-BP308637FIG
CSB-MP308637FIG
CSB-EP308637FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin-responsive protein IAA17 (IAA17) CSB-YP308638DOA
CSB-BP308638DOA
CSB-MP308638DOA
CSB-EP308638DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, chloroplastic/chromoplastic (ISPE) CSB-YP308639SZY
CSB-EP308639SZY
CSB-BP308639SZY
CSB-MP308639SZY
CSB-EP308639SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Senna didymobotrya Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP308640SFD
CSB-EP308640SFD
CSB-BP308640SFD
CSB-MP308640SFD
CSB-EP308640SFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP308641FBF
CSB-EP308641FBF
CSB-BP308641FBF
CSB-MP308641FBF
CSB-EP308641FBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative clathrin assembly protein At1g14910 (At1g14910), partial CSB-YP308642DOA
CSB-EP308642DOA
CSB-BP308642DOA
CSB-MP308642DOA
CSB-EP308642DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable phosphopantothenoylcysteine decarboxylase (HAL3B) CSB-YP308643DOA
CSB-EP308643DOA
CSB-BP308643DOA
CSB-MP308643DOA
CSB-EP308643DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae 60 kDa chaperonin (groL), partial CSB-YP308644AXG
CSB-EP308644AXG
CSB-BP308644AXG
CSB-MP308644AXG
CSB-EP308644AXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes xylosoxydans xylosoxydans N-acyl-D-glutamate deacylase CSB-YP308645AGR
CSB-EP308645AGR
CSB-BP308645AGR
CSB-MP308645AGR
CSB-EP308645AGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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