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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP308768SKY
CSB-EP308768SKY
CSB-BP308768SKY
CSB-MP308768SKY
CSB-EP308768SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP308769SKY
CSB-EP308769SKY
CSB-BP308769SKY
CSB-MP308769SKY
CSB-EP308769SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP308770SKY
CSB-EP308770SKY
CSB-BP308770SKY
CSB-MP308770SKY
CSB-EP308770SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 4 Gene 8 protein (8) CSB-YP308771EFR
CSB-EP308771EFR
CSB-BP308771EFR
CSB-MP308771EFR
CSB-EP308771EFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces capsulatus Heat shock 70 kDa protein (HSP70), partial CSB-YP308772AZC
CSB-EP308772AZC
CSB-BP308772AZC
CSB-MP308772AZC
CSB-EP308772AZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Elongation factor 1-alpha (MEF-1) CSB-YP308773EWT
CSB-EP308773EWT
CSB-BP308773EWT
CSB-MP308773EWT
CSB-EP308773EWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Probable ATP-dependent helicase 25 (25) CSB-YP308774IAD
CSB-EP308774IAD
CSB-BP308774IAD
CSB-MP308774IAD
CSB-EP308774IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Probable major glycoprotein (ORF46), partial CSB-YP308775IAD
CSB-EP308775IAD
CSB-BP308775IAD
CSB-MP308775IAD
CSB-EP308775IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF61 (ORF61) CSB-YP308776IAD
CSB-EP308776IAD
CSB-BP308776IAD
CSB-MP308776IAD
CSB-EP308776IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Putative membrane protein ORF59 (ORF59), partial CSB-YP308777IAD1
CSB-EP308777IAD1
CSB-BP308777IAD1
CSB-MP308777IAD1
CSB-EP308777IAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF34 (ORF34) CSB-YP308778IAD
CSB-EP308778IAD
CSB-BP308778IAD
CSB-MP308778IAD
CSB-EP308778IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF63 (ORF63), partial CSB-YP308779IAD
CSB-EP308779IAD
CSB-BP308779IAD
CSB-MP308779IAD
CSB-EP308779IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurid herpesvirus 1 Uncharacterized protein ORF13 (ORF13) CSB-YP308780IAD
CSB-EP308780IAD
CSB-BP308780IAD
CSB-MP308780IAD
CSB-EP308780IAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Endo-1,4-beta-xylanase C (xlnC) CSB-YP308781EKF
CSB-EP308781EKF
CSB-BP308781EKF
CSB-MP308781EKF
CSB-EP308781EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein traI (traI) CSB-YP308782ENL
CSB-EP308782ENL
CSB-BP308782ENL
CSB-MP308782ENL
CSB-EP308782ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Styela plicata Actin, muscle CSB-YP308783SQW
CSB-EP308783SQW
CSB-BP308783SQW
CSB-MP308783SQW
CSB-EP308783SQW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Putative gastrointestinal growth factor xP4 (p4) CSB-YP308784XBE
CSB-EP308784XBE
CSB-BP308784XBE
CSB-MP308784XBE
CSB-EP308784XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen fixation protein nifU (nifU) CSB-YP308785EWY
CSB-EP308785EWY
CSB-BP308785EWY
CSB-MP308785EWY
CSB-EP308785EWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucurbita maxima 1-aminocyclopropane-1-carboxylate synthase CMA101 (ACS2) CSB-YP308786DZK
CSB-EP308786DZK
CSB-BP308786DZK
CSB-MP308786DZK
CSB-EP308786DZK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP308787CUB
CSB-EP308787CUB
CSB-BP308787CUB
CSB-MP308787CUB
CSB-EP308787CUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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