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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium meliloti Catalase A (katA) CSB-YP310590RKU
CSB-EP310590RKU
CSB-BP310590RKU
CSB-MP310590RKU
CSB-EP310590RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodovulum sulfidophilum Light-harvesting protein B-800/850 alpha chain (pucA), partial CSB-YP310591RMF
CSB-EP310591RMF
CSB-BP310591RMF
CSB-MP310591RMF
CSB-EP310591RMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus 10 kDa chaperonin (groS) CSB-YP310592RLC
CSB-EP310592RLC
CSB-BP310592RLC
CSB-MP310592RLC
CSB-EP310592RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-1-phosphate thymidylyltransferase (rmlA) CSB-YP310593FMR
CSB-EP310593FMR
CSB-BP310593FMR
CSB-MP310593FMR
CSB-EP310593FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP310594FPY
CSB-EP310594FPY
CSB-BP310594FPY
CSB-MP310594FPY
CSB-EP310594FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Malto-oligosyltrehalose trehalohydrolase (treZ), partial CSB-YP310595FPM
CSB-EP310595FPM
CSB-BP310595FPM
CSB-MP310595FPM
CSB-EP310595FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Uncharacterized aminotransferase SSO0104 (SSO0104) CSB-YP310596FPM
CSB-EP310596FPM
CSB-BP310596FPM
CSB-MP310596FPM
CSB-EP310596FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus DNA polymerase 3 (dpo3), partial CSB-YP310597FPM
CSB-EP310597FPM
CSB-BP310597FPM
CSB-MP310597FPM
CSB-EP310597FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus DNA-directed RNA polymerase subunit D (rpoD) CSB-YP310598FPM
CSB-EP310598FPM
CSB-BP310598FPM
CSB-MP310598FPM
CSB-EP310598FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP310599FPM
CSB-EP310599FPM
CSB-BP310599FPM
CSB-MP310599FPM
CSB-EP310599FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Putative transcription antitermination protein NusG (nusG) CSB-YP310600FPM
CSB-EP310600FPM
CSB-BP310600FPM
CSB-MP310600FPM
CSB-EP310600FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus 50S ribosomal protein L12P (rpl12p) CSB-YP310601FPM
CSB-EP310601FPM
CSB-BP310601FPM
CSB-MP310601FPM
CSB-EP310601FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Alanine--tRNA ligase (alaS), partial CSB-YP310602FPM
CSB-EP310602FPM
CSB-BP310602FPM
CSB-MP310602FPM
CSB-EP310602FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP310603SXB
CSB-EP310603SXB
CSB-BP310603SXB
CSB-MP310603SXB
CSB-EP310603SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Putative 2-aminoethylphosphonate transport system permease protein phnV (phnV), partial CSB-YP310604SXB1
CSB-EP310604SXB1
CSB-BP310604SXB1
CSB-MP310604SXB1
CSB-EP310604SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein pyrR (pyrR) CSB-YP310605TNN
CSB-EP310605TNN
CSB-BP310605TNN
CSB-MP310605TNN
CSB-EP310605TNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP310606TPR
CSB-EP310606TPR
CSB-BP310606TPR
CSB-MP310606TPR
CSB-EP310606TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Chemotaxis protein CheY (cheY) CSB-YP310607TPR
CSB-EP310607TPR
CSB-BP310607TPR
CSB-MP310607TPR
CSB-EP310607TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum ATP-dependent DNA helicase recG (recG), partial CSB-YP310608TPR
CSB-EP310608TPR
CSB-BP310608TPR
CSB-MP310608TPR
CSB-EP310608TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Glutamate N-acetyltransferase (argJ) CSB-YP310609TNS
CSB-EP310609TNS
CSB-BP310609TNS
CSB-MP310609TNS
CSB-EP310609TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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