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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YEL075W-A (YEL075W-A) CSB-YP308970SVG
CSB-EP308970SVG
CSB-BP308970SVG
CSB-MP308970SVG
CSB-EP308970SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 8 type P E3 ubiquitin-protein ligase MIR1 (K3), partial CSB-YP308971HKE
CSB-EP308971HKE
CSB-BP308971HKE
CSB-MP308971HKE
CSB-EP308971HKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physarum polycephalum 66 kDa stress protein, partial CSB-YP308972EVC
CSB-EP308972EVC
CSB-BP308972EVC
CSB-MP308972EVC
CSB-EP308972EVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ectonucleotide pyrophosphatase/phosphodiesterase C27A7.3 (C27A7.3), partial CSB-YP308973CXY
CSB-EP308973CXY
CSB-BP308973CXY
CSB-MP308973CXY
CSB-EP308973CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class epsilon-32 (sre-32), partial CSB-YP308974CXY1
CSB-EP308974CXY1
CSB-BP308974CXY1
CSB-MP308974CXY1
CSB-EP308974CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble guanylate cyclase gcy-33 (gcy-33), partial CSB-YP308975CXY
CSB-EP308975CXY
CSB-BP308975CXY
CSB-MP308975CXY
CSB-EP308975CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class delta-31 (srd-31), partial CSB-YP308976CXY1
CSB-EP308976CXY1
CSB-BP308976CXY1
CSB-MP308976CXY1
CSB-EP308976CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuticle collagen dpy-5 (dpy-5) CSB-YP308977CXY
CSB-EP308977CXY
CSB-BP308977CXY
CSB-MP308977CXY
CSB-EP308977CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 39S ribosomal protein L24, mitochondrial (F59A3.3), partial CSB-YP308978CXY
CSB-EP308978CXY
CSB-BP308978CXY
CSB-MP308978CXY
CSB-EP308978CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase homolog 1 (T01D1.4) CSB-YP308979CXY
CSB-EP308979CXY
CSB-BP308979CXY
CSB-MP308979CXY
CSB-EP308979CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable DNA-directed RNA polymerase III subunit RPC6 (W09C3.4) CSB-YP308980CXY
CSB-EP308980CXY
CSB-BP308980CXY
CSB-MP308980CXY
CSB-EP308980CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemonchus contortus Glutamate-gated chloride channel subunit beta, partial CSB-YP308981HSW
CSB-EP308981HSW
CSB-BP308981HSW
CSB-MP308981HSW
CSB-EP308981HSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemicentrotus pulcherrimus Peptidyl-prolyl cis-trans isomerase CSB-YP308982HVL
CSB-EP308982HVL
CSB-BP308982HVL
CSB-MP308982HVL
CSB-EP308982HVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuclear hormone receptor family member nhr-69 (nhr-69) CSB-YP308983CXY
CSB-EP308983CXY
CSB-BP308983CXY
CSB-MP308983CXY
CSB-EP308983CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein F26H9.4 (F26H9.4) CSB-YP308984CXY
CSB-EP308984CXY
CSB-BP308984CXY
CSB-MP308984CXY
CSB-EP308984CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (ND42) CSB-YP308985DLU
CSB-EP308985DLU
CSB-BP308985DLU
CSB-MP308985DLU
CSB-EP308985DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litopenaeus vannamei Pigment-dispersing hormone type 1 (PDH1) CSB-YP308986LHD
CSB-EP308986LHD
CSB-BP308986LHD
CSB-MP308986LHD
CSB-EP308986LHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Variant-specific surface protein VSP4A1, partial CSB-YP308987GFW
CSB-EP308987GFW
CSB-BP308987GFW
CSB-MP308987GFW
CSB-EP308987GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein lin-28 (lin-28) CSB-YP308988CXY
CSB-EP308988CXY
CSB-BP308988CXY
CSB-MP308988CXY
CSB-EP308988CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP308989CSH
CSB-EP308989CSH
CSB-BP308989CSH
CSB-MP308989CSH
CSB-EP308989CSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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