Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermus thermophilus Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP308727TNS
CSB-EP308727TNS
CSB-BP308727TNS
CSB-MP308727TNS
CSB-EP308727TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized methyltransferase ydaC (ydaC) CSB-YP308729BRJ
CSB-EP308729BRJ
CSB-BP308729BRJ
CSB-MP308729BRJ
CSB-EP308729BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable C4-dicarboxylate response regulator dctR (dctR) CSB-YP308730BRJ
CSB-EP308730BRJ
CSB-BP308730BRJ
CSB-MP308730BRJ
CSB-EP308730BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydbL (ydbL), partial CSB-YP308731BRJ1
CSB-EP308731BRJ1
CSB-BP308731BRJ1
CSB-MP308731BRJ1
CSB-EP308731BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transporter ydbO (ydbO), partial CSB-YP308732BRJ1
CSB-EP308732BRJ1
CSB-BP308732BRJ1
CSB-MP308732BRJ1
CSB-EP308732BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Antitoxin EndoAI (ndoAI) CSB-YP308733BRJ
CSB-EP308733BRJ
CSB-BP308733BRJ
CSB-MP308733BRJ
CSB-EP308733BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddC (yddC), partial CSB-YP308734BRJ1
CSB-EP308734BRJ1
CSB-BP308734BRJ1
CSB-MP308734BRJ1
CSB-EP308734BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized isochorismatase family protein yddQ (yddQ) CSB-YP308735BRJ
CSB-EP308735BRJ
CSB-BP308735BRJ
CSB-MP308735BRJ
CSB-EP308735BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter ydeG (ydeG), partial CSB-YP308736BRJ
CSB-EP308736BRJ
CSB-BP308736BRJ
CSB-MP308736BRJ
CSB-EP308736BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0750 membrane protein ydeO (ydeO), partial CSB-YP308737BRJ1
CSB-EP308737BRJ1
CSB-BP308737BRJ1
CSB-MP308737BRJ1
CSB-EP308737BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative arsenical pump membrane protein YdfA (ydfA), partial CSB-YP308738BRJ
CSB-EP308738BRJ
CSB-BP308738BRJ
CSB-MP308738BRJ
CSB-EP308738BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulatory protein ydfI (ydfI) CSB-YP308739BRJ
CSB-EP308739BRJ
CSB-BP308739BRJ
CSB-MP308739BRJ
CSB-EP308739BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydfL (ydfL) CSB-YP308740BRJ
CSB-EP308740BRJ
CSB-BP308740BRJ
CSB-MP308740BRJ
CSB-EP308740BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydgA (ydgA) CSB-YP308741BRJ
CSB-EP308741BRJ
CSB-BP308741BRJ
CSB-MP308741BRJ
CSB-EP308741BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydgJ (ydgJ) CSB-YP308742BRJ
CSB-EP308742BRJ
CSB-BP308742BRJ
CSB-MP308742BRJ
CSB-EP308742BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable capsular polysaccharide biosynthesis protein YwqC (ywqC), partial CSB-YP308743BRJ1
CSB-EP308743BRJ1
CSB-BP308743BRJ1
CSB-MP308743BRJ1
CSB-EP308743BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell wall-binding protein ywsB (ywsB) CSB-YP308744BRJ
CSB-EP308744BRJ
CSB-BP308744BRJ
CSB-MP308744BRJ
CSB-EP308744BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Putative potassium transport system protein kup 1 (kup1), partial CSB-YP308745LND
CSB-EP308745LND
CSB-BP308745LND
CSB-MP308745LND
CSB-EP308745LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0146 (Rv0146, MT0154) CSB-YP308746MVZ
CSB-EP308746MVZ
CSB-BP308746MVZ
CSB-MP308746MVZ
CSB-EP308746MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Iron-dependent extradiol dioxygenase (hsaC) CSB-YP308747MVZ
CSB-EP308747MVZ
CSB-BP308747MVZ
CSB-MP308747MVZ
CSB-EP308747MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>