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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Probable DNA ligase (lig), partial CSB-YP308687MVZ
CSB-EP308687MVZ
CSB-BP308687MVZ
CSB-MP308687MVZ
CSB-EP308687MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase (Rv2951c, MT3025) CSB-YP308688MVZ
CSB-EP308688MVZ
CSB-BP308688MVZ
CSB-MP308688MVZ
CSB-EP308688MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative NAD (P)H nitroreductase RV3131/MT3217 CSB-YP308689MVZ
CSB-EP308689MVZ
CSB-BP308689MVZ
CSB-MP308689MVZ
CSB-EP308689MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ESAT-6-like protein 6 (Rv2346c, MT2411) CSB-YP308690MVZ
CSB-EP308690MVZ
CSB-BP308690MVZ
CSB-MP308690MVZ
CSB-EP308690MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB14 (vapB14) CSB-YP308691MVZ
CSB-EP308691MVZ
CSB-BP308691MVZ
CSB-MP308691MVZ
CSB-EP308691MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Flagellin B2 (flaB2) CSB-YP308692MNS
CSB-EP308692MNS
CSB-BP308692MNS
CSB-MP308692MNS
CSB-EP308692MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate dehydrogenase (aroE) CSB-YP308693NFV
CSB-EP308693NFV
CSB-BP308693NFV
CSB-MP308693NFV
CSB-EP308693NFV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate dehydrogenase (aroE) CSB-YP308694NFZ
CSB-EP308694NFZ
CSB-BP308694NFZ
CSB-MP308694NFZ
CSB-EP308694NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP308695NGG
CSB-EP308695NGG
CSB-BP308695NGG
CSB-MP308695NGG
CSB-EP308695NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate dehydrogenase (aroE) CSB-YP308696NGK
CSB-EP308696NGK
CSB-BP308696NGK
CSB-MP308696NGK
CSB-EP308696NGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Type III export protein pscG (pscG) CSB-YP308697EZX
CSB-EP308697EZX
CSB-BP308697EZX
CSB-MP308697EZX
CSB-EP308697EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus opacus Muconolactone Delta-isomerase (catC) CSB-YP308698RLM
CSB-EP308698RLM
CSB-BP308698RLM
CSB-MP308698RLM
CSB-EP308698RLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum molischianum Light-harvesting protein B-800/850 beta 1 chain (B1), partial CSB-YP308699RLZ
CSB-EP308699RLZ
CSB-BP308699RLZ
CSB-MP308699RLZ
CSB-EP308699RLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus 60 kDa chaperonin (groL), partial CSB-YP308700RLC
CSB-EP308700RLC
CSB-BP308700RLC
CSB-MP308700RLC
CSB-EP308700RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor GTPase obg (obg) CSB-YP308701FOB
CSB-EP308701FOB
CSB-BP308701FOB
CSB-MP308701FOB
CSB-EP308701FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Intracellular septation protein (yciB), partial CSB-YP308702SZB1
CSB-EP308702SZB1
CSB-BP308702SZB1
CSB-MP308702SZB1
CSB-EP308702SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant dTDP-glucose 4,6-dehydratase (rmlB) CSB-YP308703FMR
CSB-EP308703FMR
CSB-BP308703FMR
CSB-MP308703FMR
CSB-EP308703FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP308704FMR
CSB-EP308704FMR
CSB-BP308704FMR
CSB-MP308704FMR
CSB-EP308704FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP308705FMW
CSB-EP308705FMW
CSB-BP308705FMW
CSB-MP308705FMW
CSB-EP308705FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Probable peroxiredoxin (SSO2121) CSB-YP308706FPM
CSB-EP308706FPM
CSB-BP308706FPM
CSB-MP308706FPM
CSB-EP308706FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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