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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 2-isopropylmalate synthase (leuA), partial CSB-YP310569MOA
CSB-EP310569MOA
CSB-BP310569MOA
CSB-MP310569MOA
CSB-EP310569MOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Regulatory protein recX (recX) CSB-YP310570MVX
CSB-EP310570MVX
CSB-BP310570MVX
CSB-MP310570MVX
CSB-EP310570MVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB19 (vapB19) CSB-YP310572MVZ
CSB-EP310572MVZ
CSB-BP310572MVZ
CSB-MP310572MVZ
CSB-EP310572MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonuclease VapC17 (vapC17) CSB-YP310573MVZ
CSB-EP310573MVZ
CSB-BP310573MVZ
CSB-MP310573MVZ
CSB-EP310573MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis 50S ribosomal protein L2 (rplB) CSB-YP310574MVZ
CSB-BP310574MVZ
CSB-MP310574MVZ
CSB-EP310574MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serine/threonine-protein kinase pknK (pknK), partial CSB-YP310575MVZ
CSB-EP310575MVZ
CSB-BP310575MVZ
CSB-MP310575MVZ
CSB-EP310575MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thiamine-monophosphate kinase (thiL) CSB-YP310576MVZ
CSB-EP310576MVZ
CSB-BP310576MVZ
CSB-MP310576MVZ
CSB-EP310576MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative esterase Rv1847/MT1895 (Rv1847, MT1895) CSB-YP310577MVZ
CSB-EP310577MVZ
CSB-BP310577MVZ
CSB-MP310577MVZ
CSB-EP310577MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional regulatory protein devR (dosR) CSB-YP310578MVZ
CSB-EP310578MVZ
CSB-BP310578MVZ
CSB-MP310578MVZ
CSB-EP310578MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0603 protein Rv2345/MT2410 (Rv2345, MT2410), partial CSB-YP310579MVZ1
CSB-EP310579MVZ1
CSB-BP310579MVZ1
CSB-MP310579MVZ1
CSB-EP310579MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospholipase C 3 (plcC) CSB-YP310580MVZ
CSB-EP310580MVZ
CSB-BP310580MVZ
CSB-MP310580MVZ
CSB-EP310580MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antitoxin HigA (higA) CSB-YP310581MVZ
CSB-EP310581MVZ
CSB-BP310581MVZ
CSB-MP310581MVZ
CSB-EP310581MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (nifE) CSB-YP310582MSQ
CSB-EP310582MSQ
CSB-BP310582MSQ
CSB-MP310582MSQ
CSB-EP310582MSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxococcus xanthus Chaperone protein DnaK (dnaK), partial CSB-YP310583MXB
CSB-EP310583MXB
CSB-BP310583MXB
CSB-MP310583MXB
CSB-EP310583MXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Siroheme synthase (cysG1) CSB-YP310584NGG
CSB-EP310584NGG
CSB-BP310584NGG
CSB-MP310584NGG
CSB-EP310584NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP310585NGG
CSB-EP310585NGG
CSB-BP310585NGG
CSB-MP310585NGG
CSB-EP310585NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP310586EZX
CSB-EP310586EZX
CSB-BP310586EZX
CSB-MP310586EZX
CSB-EP310586EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Gingipain R2 (rgpB), partial CSB-YP310587EYA
CSB-BP310587EYA
CSB-MP310587EYA
CSB-EP310587EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Glutamyl-tRNA reductase (hemA) CSB-YP310588ESG
CSB-EP310588ESG
CSB-BP310588ESG
CSB-MP310588ESG
CSB-EP310588ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Response regulator gacA (gacA) CSB-YP310589PWH
CSB-EP310589PWH
CSB-BP310589PWH
CSB-MP310589PWH
CSB-EP310589PWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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