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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Putative cysteine protease ywpE (ywpE) CSB-YP310167BRJ
CSB-EP310167BRJ
CSB-BP310167BRJ
CSB-MP310167BRJ
CSB-EP310167BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum DNA gyrase subunit B (gyrB), partial CSB-YP310168DUB
CSB-EP310168DUB
CSB-BP310168DUB
CSB-MP310168DUB
CSB-EP310168DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Potassium-transporting ATPase C chain (kdpC), partial CSB-YP310169DUB1
CSB-EP310169DUB1
CSB-BP310169DUB1
CSB-MP310169DUB1
CSB-EP310169DUB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP310170DXP
CSB-EP310170DXP
CSB-BP310170DXP
CSB-MP310170DXP
CSB-EP310170DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Sulfur-rich protein (srp), partial CSB-YP310171DSL1
CSB-EP310171DSL1
CSB-BP310171DSL1
CSB-MP310171DSL1
CSB-EP310171DSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L27 (rpmA) CSB-YP310172CJN
CSB-EP310172CJN
CSB-BP310172CJN
CSB-MP310172CJN
CSB-EP310172CJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax lucentense Beta-galactosidase BgaH (bgaH), partial CSB-YP310173HBS
CSB-EP310173HBS
CSB-BP310173HBS
CSB-MP310173HBS
CSB-EP310173HBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori UvrABC system protein B (uvrB), partial CSB-YP310174HUV
CSB-EP310174HUV
CSB-BP310174HUV
CSB-MP310174HUV
CSB-EP310174HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyruvate kinase (pyk) CSB-YP310175MVM
CSB-EP310175MVM
CSB-BP310175MVM
CSB-MP310175MVM
CSB-EP310175MVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonuclease VapC18 (vapC18) CSB-YP310176MVZ
CSB-EP310176MVZ
CSB-BP310176MVZ
CSB-MP310176MVZ
CSB-EP310176MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N utilization substance protein B homolog (nusB) CSB-YP310177MVZ
CSB-EP310177MVZ
CSB-BP310177MVZ
CSB-MP310177MVZ
CSB-EP310177MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative mycofactocin biosynthesis transcriptional regulator MftR (mftR) CSB-YP310178MVZ
CSB-EP310178MVZ
CSB-BP310178MVZ
CSB-MP310178MVZ
CSB-EP310178MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative mycofactocin biosynthesis glycosyltransferase MftF (mftF), partial CSB-YP310179MVZ1
CSB-EP310179MVZ1
CSB-BP310179MVZ1
CSB-MP310179MVZ1
CSB-EP310179MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PGL/p-HBAD biosynthesis rhamnosyltransferase (Rv2962c, MT3038) CSB-YP310180MVZ
CSB-EP310180MVZ
CSB-BP310180MVZ
CSB-MP310180MVZ
CSB-EP310180MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum import ATP-binding protein ModC (modC) CSB-YP310181MVZ
CSB-EP310181MVZ
CSB-BP310181MVZ
CSB-MP310181MVZ
CSB-EP310181MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein mmpL1 (mmpL1), partial CSB-YP310182MVZ
CSB-EP310182MVZ
CSB-BP310182MVZ
CSB-MP310182MVZ
CSB-EP310182MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized PPE family protein PPE36 (ppe36) CSB-YP310183MVZ
CSB-EP310183MVZ
CSB-BP310183MVZ
CSB-MP310183MVZ
CSB-EP310183MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate dehydrogenase (aroE) CSB-YP310184NGC
CSB-EP310184NGC
CSB-BP310184NGC
CSB-MP310184NGC
CSB-EP310184NGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Major cold shock protein CspA (cspA) CSB-YP310185EZX
CSB-EP310185EZX
CSB-BP310185EZX
CSB-MP310185EZX
CSB-EP310185EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP310186PBZ
CSB-EP310186PBZ
CSB-BP310186PBZ
CSB-MP310186PBZ
CSB-EP310186PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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