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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein ysfE (ysfE) CSB-YP309051BRJ
CSB-EP309051BRJ
CSB-BP309051BRJ
CSB-MP309051BRJ
CSB-EP309051BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transmembrane protein yshB (yshB), partial CSB-YP309052BRJ1
CSB-EP309052BRJ1
CSB-BP309052BRJ1
CSB-MP309052BRJ1
CSB-EP309052BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Long-chain-fatty-acid--CoA ligase (lcfA), partial CSB-YP309053BRJ
CSB-EP309053BRJ
CSB-BP309053BRJ
CSB-MP309053BRJ
CSB-EP309053BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative metallophosphoesterase ysnB (ysnB) CSB-YP309054BRJ
CSB-EP309054BRJ
CSB-BP309054BRJ
CSB-MP309054BRJ
CSB-EP309054BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized isochorismatase family protein ywoC (ywoC) CSB-YP309055BRJ
CSB-EP309055BRJ
CSB-BP309055BRJ
CSB-MP309055BRJ
CSB-EP309055BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywpG (ywpG) CSB-YP309056BRJ
CSB-EP309056BRJ
CSB-BP309056BRJ
CSB-MP309056BRJ
CSB-EP309056BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized phosphatase YwpJ (ywpJ) CSB-YP309057BRJ
CSB-EP309057BRJ
CSB-BP309057BRJ
CSB-MP309057BRJ
CSB-EP309057BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Early upstream open reading frame (CCA_00181) CSB-YP309058DSL
CSB-EP309058DSL
CSB-BP309058DSL
CSB-MP309058DSL
CSB-EP309058DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter agglomerans Mercuric reductase (merA), partial CSB-YP309059EKP
CSB-EP309059EKP
CSB-BP309059EKP
CSB-MP309059EKP
CSB-EP309059EKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans 50S ribosomal protein L3 (rplC) CSB-YP309060AYK
CSB-EP309060AYK
CSB-BP309060AYK
CSB-MP309060AYK
CSB-EP309060AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aminopeptidase G (pepG) CSB-YP309061LAS
CSB-EP309061LAS
CSB-BP309061LAS
CSB-MP309061LAS
CSB-EP309061LAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanopyrus kandleri Uncharacterized protein MK0008 (MK0008) CSB-YP309062MSI
CSB-EP309062MSI
CSB-BP309062MSI
CSB-MP309062MSI
CSB-EP309062MSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacillus sp. Biotin synthase (bioB) CSB-YP309063MFI
CSB-EP309063MFI
CSB-BP309063MFI
CSB-MP309063MFI
CSB-EP309063MFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP309064MVZ
CSB-EP309064MVZ
CSB-BP309064MVZ
CSB-MP309064MVZ
CSB-EP309064MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Putative lipoprotein lppB (lppB) CSB-YP309065MVZ
CSB-EP309065MVZ
CSB-BP309065MVZ
CSB-MP309065MVZ
CSB-EP309065MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Rv0726c/MT0751 (Rv0726c, MT0751) CSB-YP309066MVZ
CSB-EP309066MVZ
CSB-BP309066MVZ
CSB-MP309066MVZ
CSB-EP309066MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 8-oxo-dGTP diphosphatase 1 (mutT1) CSB-YP309067MVZ
CSB-EP309067MVZ
CSB-BP309067MVZ
CSB-MP309067MVZ
CSB-EP309067MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase Rv2971/MT3049 (Rv2971, MT3049) CSB-YP309068MVZ
CSB-EP309068MVZ
CSB-BP309068MVZ
CSB-MP309068MVZ
CSB-EP309068MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Universal stress protein Rv3134c/MT3220 (Rv3134c, MT3220) CSB-YP309069MVZ
CSB-EP309069MVZ
CSB-BP309069MVZ
CSB-MP309069MVZ
CSB-EP309069MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--AMP ligase FadD30 (fadD30), partial CSB-YP309070MVZ
CSB-EP309070MVZ
CSB-BP309070MVZ
CSB-MP309070MVZ
CSB-EP309070MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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