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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis PGA biosynthesis protein CapC (capC) CSB-YP309131BRJ
CSB-EP309131BRJ
CSB-BP309131BRJ
CSB-MP309131BRJ
CSB-EP309131BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans UPF0042 nucleotide-binding protein HI1146 homolog CSB-YP309132AYK
CSB-EP309132AYK
CSB-BP309132AYK
CSB-MP309132AYK
CSB-EP309132AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized tRNA/rRNA methyltransferase Rv3579c/MT3685 (Rv3579c, MT3685) CSB-YP309133MVZ
CSB-EP309133MVZ
CSB-BP309133MVZ
CSB-MP309133MVZ
CSB-EP309133MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N5-carboxyaminoimidazole ribonucleotide mutase (purE) CSB-YP309134MVZ
CSB-EP309134MVZ
CSB-BP309134MVZ
CSB-MP309134MVZ
CSB-EP309134MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease V alpha chain (recD), partial CSB-YP309135MVZ
CSB-EP309135MVZ
CSB-BP309135MVZ
CSB-MP309135MVZ
CSB-EP309135MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0336 protein Rv0637/MT0666 (Rv0637, MT0666) CSB-YP309136MVZ
CSB-EP309136MVZ
CSB-BP309136MVZ
CSB-MP309136MVZ
CSB-EP309136MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Protein RnfH (rnfH) CSB-YP309137RLC
CSB-EP309137RLC
CSB-BP309137RLC
CSB-MP309137RLC
CSB-EP309137RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Motility protein A (motA), partial CSB-YP309138RKU1
CSB-EP309138RKU1
CSB-BP309138RKU1
CSB-MP309138RKU1
CSB-EP309138RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori CAG pathogenicity island protein 12 (cagT) CSB-YP309139HUV
CSB-EP309139HUV
CSB-BP309139HUV
CSB-MP309139HUV
CSB-EP309139HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bryonia dioica Ribosome-inactivating protein bryodin II CSB-YP309141BMR
CSB-EP309141BMR
CSB-BP309141BMR
CSB-MP309141BMR
CSB-EP309141BMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Unknown protein from spot 2D-0014LD of 2D-PAGE of liver tissue CSB-YP309142MO
CSB-EP309142MO
CSB-BP309142MO
CSB-MP309142MO
CSB-EP309142MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein grpE (grpE) CSB-YP309143SKY
CSB-EP309143SKY
CSB-BP309143SKY
CSB-MP309143SKY
CSB-EP309143SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphopentomutase (deoB) CSB-YP309144SKY
CSB-EP309144SKY
CSB-BP309144SKY
CSB-MP309144SKY
CSB-EP309144SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP309145SKY
CSB-EP309145SKY
CSB-BP309145SKY
CSB-MP309145SKY
CSB-EP309145SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glucosamine-6-phosphate deaminase (nagB) CSB-YP309146SKY
CSB-EP309146SKY
CSB-BP309146SKY
CSB-MP309146SKY
CSB-EP309146SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoglycerate kinase (pgk) CSB-YP309147SKY
CSB-EP309147SKY
CSB-BP309147SKY
CSB-MP309147SKY
CSB-EP309147SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0173 metal-dependent hydrolase SA1529 (SA1529) CSB-YP309148SKY
CSB-EP309148SKY
CSB-BP309148SKY
CSB-MP309148SKY
CSB-EP309148SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Deoxyribose-phosphate aldolase 2 (deoC2) CSB-YP309149SKY
CSB-EP309149SKY
CSB-BP309149SKY
CSB-MP309149SKY
CSB-EP309149SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhamnogalacturonase A (rhgA) CSB-YP309150DOV
CSB-EP309150DOV
CSB-BP309150DOV
CSB-MP309150DOV
CSB-EP309150DOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley yellow dwarf virus Major capsid protein (ORF3) CSB-YP309151BEQ
CSB-EP309151BEQ
CSB-BP309151BEQ
CSB-MP309151BEQ
CSB-EP309151BEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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