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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human herpesvirus 2 Triplex capsid protein VP23 (UL18) CSB-YP309334HJX
CSB-EP309334HJX
CSB-BP309334HJX
CSB-MP309334HJX
CSB-EP309334HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Virion-packaging protein UL25 (UL25), partial CSB-YP309335HJX
CSB-EP309335HJX
CSB-BP309335HJX
CSB-MP309335HJX
CSB-EP309335HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 DNA primase (UL52), partial CSB-YP309336HJX
CSB-EP309336HJX
CSB-BP309336HJX
CSB-MP309336HJX
CSB-EP309336HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crimean-Congo hemorrhagic fever virus Nucleoprotein (N) CSB-YP309337CSC
CSB-EP309337CSC
CSB-BP309337CSC
CSB-MP309337CSC
CSB-EP309337CSC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicular exanthema of swine virus serotype A48 Protein VP2 (ORF3) CSB-YP309338VBC
CSB-EP309338VBC
CSB-BP309338VBC
CSB-MP309338VBC
CSB-EP309338VBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus OPEP-3 protein (OPEP-3) CSB-YP309339OEU
CSB-EP309339OEU
CSB-BP309339OEU
CSB-MP309339OEU
CSB-EP309339OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase [NAD (+)], glycosomal CSB-YP309340LDQ
CSB-EP309340LDQ
CSB-BP309340LDQ
CSB-MP309340LDQ
CSB-EP309340LDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ectonucleotide pyrophosphatase/phosphodiesterase C27A7.1 (C27A7.1), partial CSB-YP309341CXY
CSB-EP309341CXY
CSB-BP309341CXY
CSB-MP309341CXY
CSB-EP309341CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant E3 ubiquitin-protein ligase hrd-like protein 1 (hrdl-1), partial CSB-YP309342CXY
CSB-EP309342CXY
CSB-BP309342CXY
CSB-MP309342CXY
CSB-EP309342CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Intermediate filament protein ifa-1 (ifa-1), partial CSB-YP309343CXY
CSB-EP309343CXY
CSB-BP309343CXY
CSB-MP309343CXY
CSB-EP309343CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Golgin-84 (T24B1.1), partial CSB-YP309344CXY
CSB-EP309344CXY
CSB-BP309344CXY
CSB-MP309344CXY
CSB-EP309344CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant E3 ubiquitin-protein ligase ubr-1 (ubr-1), partial CSB-YP309345CXY
CSB-EP309345CXY
CSB-BP309345CXY
CSB-MP309345CXY
CSB-EP309345CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serpentine receptor class delta-30 (srd-30), partial CSB-YP309346CXY1
CSB-EP309346CXY1
CSB-BP309346CXY1
CSB-MP309346CXY1
CSB-EP309346CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Structure-specific endonuclease subunit SLX1 homolog (giyd-1) CSB-YP309347CXY
CSB-EP309347CXY
CSB-BP309347CXY
CSB-MP309347CXY
CSB-EP309347CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative syntaxin-4 (syn-4), partial CSB-YP309348CXY
CSB-EP309348CXY
CSB-BP309348CXY
CSB-MP309348CXY
CSB-EP309348CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sperm-specific class P protein 16 (ssp-16) CSB-YP309349CXY
CSB-EP309349CXY
CSB-BP309349CXY
CSB-MP309349CXY
CSB-EP309349CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila capricorni Period circadian protein (per) CSB-YP309350DFW
CSB-EP309350DFW
CSB-BP309350DFW
CSB-MP309350DFW
CSB-EP309350DFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Opsin Rh5 (Rh5), partial CSB-YP309351DLU1
CSB-EP309351DLU1
CSB-BP309351DLU1
CSB-MP309351DLU1
CSB-EP309351DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Metabotropic glutamate receptor (mGluRA), partial CSB-YP309352DLU
CSB-EP309352DLU
CSB-BP309352DLU
CSB-MP309352DLU
CSB-EP309352DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein decapentaplegic (dpp) CSB-YP309353DMJ
CSB-EP309353DMJ
CSB-BP309353DMJ
CSB-MP309353DMJ
CSB-EP309353DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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