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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Sensor protein lytS (lytS), partial CSB-YP310955BRJ1
CSB-EP310955BRJ1
CSB-BP310955BRJ1
CSB-MP310955BRJ1
CSB-EP310955BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yscB (yscB) CSB-YP310956BRJ
CSB-EP310956BRJ
CSB-BP310956BRJ
CSB-MP310956BRJ
CSB-EP310956BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP310957BRJ
CSB-EP310957BRJ
CSB-BP310957BRJ
CSB-MP310957BRJ
CSB-EP310957BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycolate oxidase subunit glcD (glcD) CSB-YP310958BRJ
CSB-EP310958BRJ
CSB-BP310958BRJ
CSB-MP310958BRJ
CSB-EP310958BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division protein ZapA (zapA) CSB-YP310959BRJ
CSB-EP310959BRJ
CSB-BP310959BRJ
CSB-MP310959BRJ
CSB-EP310959BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamate racemase 1 (racE) CSB-YP310960BRJ
CSB-EP310960BRJ
CSB-BP310960BRJ
CSB-MP310960BRJ
CSB-EP310960BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 2-isopropylmalate synthase (leuA), partial CSB-YP310961BRJ
CSB-EP310961BRJ
CSB-BP310961BRJ
CSB-MP310961BRJ
CSB-EP310961BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Undecaprenyl-diphosphatase BcrC (bcrC), partial CSB-YP310962BRJ1
CSB-EP310962BRJ1
CSB-BP310962BRJ1
CSB-MP310962BRJ1
CSB-EP310962BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable allantoin permease (pucI), partial CSB-YP310963BRJ
CSB-EP310963BRJ
CSB-BP310963BRJ
CSB-MP310963BRJ
CSB-EP310963BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Large cysteine-rich periplasmic protein omcB (omcB), partial CSB-YP310964DSL
CSB-EP310964DSL
CSB-BP310964DSL
CSB-MP310964DSL
CSB-EP310964DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L21 (rplU) CSB-YP310965CJN
CSB-EP310965CJN
CSB-BP310965CJN
CSB-MP310965CJN
CSB-EP310965CJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP310966HHO
CSB-EP310966HHO
CSB-BP310966HHO
CSB-MP310966HHO
CSB-EP310966HHO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 36 kDa antigen, partial CSB-YP310967HUV1
CSB-EP310967HUV1
CSB-BP310967HUV1
CSB-MP310967HUV1
CSB-EP310967HUV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonuclease VapC19 (vapC19) CSB-YP310969MVZ
CSB-EP310969MVZ
CSB-BP310969MVZ
CSB-MP310969MVZ
CSB-EP310969MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative lipoprotein lppA (lppA) CSB-YP310970MVZ
CSB-EP310970MVZ
CSB-BP310970MVZ
CSB-MP310970MVZ
CSB-EP310970MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L15 (rplO) CSB-YP310971MVZ
CSB-EP310971MVZ
CSB-BP310971MVZ
CSB-MP310971MVZ
CSB-EP310971MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP310972MVZ
CSB-EP310972MVZ
CSB-BP310972MVZ
CSB-MP310972MVZ
CSB-EP310972MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant PGL/p-HBAD biosynthesis glycosyltransferase Rv2958c/MT3034 (Rv2958c, MT3034) CSB-YP310973MVZ
CSB-EP310973MVZ
CSB-BP310973MVZ
CSB-MP310973MVZ
CSB-EP310973MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized response regulatory protein Rv3143/MT3230 (Rv3143, MT3230) CSB-YP310974MVZ
CSB-EP310974MVZ
CSB-BP310974MVZ
CSB-MP310974MVZ
CSB-EP310974MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonuclease VapC35 (vapC35) CSB-YP310975MVZ
CSB-EP310975MVZ
CSB-BP310975MVZ
CSB-MP310975MVZ
CSB-EP310975MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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