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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Negative regulatory protein yxlE (yxlE), partial CSB-YP310146BRJ1
CSB-EP310146BRJ1
CSB-BP310146BRJ1
CSB-MP310146BRJ1
CSB-EP310146BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative 3-methyladenine DNA glycosylase (yxlJ) CSB-YP310147BRJ
CSB-EP310147BRJ
CSB-BP310147BRJ
CSB-MP310147BRJ
CSB-EP310147BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YcgP (ycgP) CSB-YP310148BRJ
CSB-EP310148BRJ
CSB-BP310148BRJ
CSB-MP310148BRJ
CSB-EP310148BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GTP cyclohydrolase folE2 (folE2) CSB-YP310149BRJ
CSB-EP310149BRJ
CSB-BP310149BRJ
CSB-MP310149BRJ
CSB-EP310149BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YclH (yclH) CSB-YP310150BRJ
CSB-EP310150BRJ
CSB-BP310150BRJ
CSB-MP310150BRJ
CSB-EP310150BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphatase rapC inhibitor (phrC) CSB-YP310151BRJ
CSB-EP310151BRJ
CSB-BP310151BRJ
CSB-MP310151BRJ
CSB-EP310151BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ycnC (ycnC) CSB-YP310152BRJ
CSB-EP310152BRJ
CSB-BP310152BRJ
CSB-MP310152BRJ
CSB-EP310152BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase YfiI (yfiI) CSB-YP310153BRJ
CSB-EP310153BRJ
CSB-BP310153BRJ
CSB-MP310153BRJ
CSB-EP310153BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transport permease yfiM (yfiM), partial CSB-YP310154BRJ1
CSB-EP310154BRJ1
CSB-BP310154BRJ1
CSB-MP310154BRJ1
CSB-EP310154BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yeaC (yeaC) CSB-YP310156BRJ
CSB-EP310156BRJ
CSB-BP310156BRJ
CSB-MP310156BRJ
CSB-EP310156BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ynaE (ynaE) CSB-YP310157BRJ
CSB-EP310157BRJ
CSB-BP310157BRJ
CSB-MP310157BRJ
CSB-EP310157BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ynaI (ynaI) CSB-YP310158BRJ
CSB-EP310158BRJ
CSB-BP310158BRJ
CSB-MP310158BRJ
CSB-EP310158BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation protein cse60 (cse60) CSB-YP310159BRJ
CSB-EP310159BRJ
CSB-BP310159BRJ
CSB-MP310159BRJ
CSB-EP310159BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulatory protein yvrH (yvrH) CSB-YP310160BRJ
CSB-EP310160BRJ
CSB-BP310160BRJ
CSB-MP310160BRJ
CSB-EP310160BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensory transduction protein lytT (lytT) CSB-YP310161BRJ
CSB-EP310161BRJ
CSB-BP310161BRJ
CSB-MP310161BRJ
CSB-EP310161BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-arabinose isomerase (araA) CSB-YP310162BRJ
CSB-BP310162BRJ
CSB-MP310162BRJ
CSB-EP310162BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis L-arabinose transport system permease protein AraP (araP), partial CSB-YP310163BRJ1
CSB-EP310163BRJ1
CSB-BP310163BRJ1
CSB-MP310163BRJ1
CSB-EP310163BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Ribonuclease HIII (rnhC) CSB-YP310164BRJ
CSB-EP310164BRJ
CSB-BP310164BRJ
CSB-MP310164BRJ
CSB-EP310164BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YsnE (ysnE) CSB-YP310165BRJ
CSB-EP310165BRJ
CSB-BP310165BRJ
CSB-MP310165BRJ
CSB-EP310165BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter ywoD (ywoD), partial CSB-YP310166BRJ
CSB-EP310166BRJ
CSB-BP310166BRJ
CSB-MP310166BRJ
CSB-EP310166BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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