Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Protein RPN4 (RPN4), partial CSB-YP311782SVG
CSB-EP311782SVG
CSB-BP311782SVG
CSB-MP311782SVG
CSB-EP311782SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar protein LafL (lafL), partial CSB-YP311783VFE
CSB-EP311783VFE
CSB-BP311783VFE
CSB-MP311783VFE
CSB-EP311783VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Myosin IE heavy chain (myoE), partial CSB-YP311784DKK
CSB-EP311784DKK
CSB-BP311784DKK
CSB-MP311784DKK
CSB-EP311784DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase STE20 (STE20), partial CSB-YP311785SVG
CSB-EP311785SVG
CSB-BP311785SVG
CSB-MP311785SVG
CSB-EP311785SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 6-phospho-alpha-glucosidase 2 (LSEI_2684) CSB-YP311786LAN
CSB-EP311786LAN
CSB-BP311786LAN
CSB-MP311786LAN
CSB-EP311786LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP311787LAN
CSB-EP311787LAN
CSB-BP311787LAN
CSB-MP311787LAN
CSB-EP311787LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L3 (rplC) CSB-YP311788LAN
CSB-EP311788LAN
CSB-BP311788LAN
CSB-MP311788LAN
CSB-EP311788LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S19 (rpsS) CSB-YP311789LAN
CSB-EP311789LAN
CSB-BP311789LAN
CSB-MP311789LAN
CSB-EP311789LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S17 (rpsQ) CSB-YP311790LAN
CSB-EP311790LAN
CSB-BP311790LAN
CSB-MP311790LAN
CSB-EP311790LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L18 (rplR) CSB-YP311791LAN
CSB-EP311791LAN
CSB-BP311791LAN
CSB-MP311791LAN
CSB-EP311791LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YMR265C (YMR265C) CSB-YP311792SVG
CSB-EP311792SVG
CSB-BP311792SVG
CSB-MP311792SVG
CSB-EP311792SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP311793BRJ
CSB-EP311793BRJ
CSB-BP311793BRJ
CSB-MP311793BRJ
CSB-EP311793BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P22 Ead protein (ead) CSB-YP311794EDG
CSB-EP311794EDG
CSB-BP311794EDG
CSB-MP311794EDG
CSB-EP311794EDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine adenovirus B serotype 3 Fiber protein (PIV), partial CSB-YP311795BVG
CSB-EP311795BVG
CSB-BP311795BVG
CSB-MP311795BVG
CSB-EP311795BVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glutamyl-tRNA (Gln) amidotransferase subunit A, mitochondrial CSB-YP311796SVG
CSB-EP311796SVG
CSB-BP311796SVG
CSB-MP311796SVG
CSB-EP311796SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein B0464.6 (B0464.6), partial CSB-YP311797CXY
CSB-EP311797CXY
CSB-BP311797CXY
CSB-MP311797CXY
CSB-EP311797CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Flagellar motor switch protein FliN (fliN) CSB-YP311799DQO
CSB-EP311799DQO
CSB-BP311799DQO
CSB-MP311799DQO
CSB-EP311799DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP311800LAN
CSB-EP311800LAN
CSB-BP311800LAN
CSB-MP311800LAN
CSB-EP311800LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 30S ribosomal protein S9 (rpsI) CSB-YP311801LAN
CSB-EP311801LAN
CSB-BP311801LAN
CSB-MP311801LAN
CSB-EP311801LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei 50S ribosomal protein L11 (rplK) CSB-YP311802LAN
CSB-EP311802LAN
CSB-BP311802LAN
CSB-MP311802LAN
CSB-EP311802LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>