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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas aeruginosa Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP312595PUC
CSB-EP312595PUC
CSB-BP312595PUC
CSB-MP312595PUC
CSB-EP312595PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP312596PUC
CSB-EP312596PUC
CSB-BP312596PUC
CSB-MP312596PUC
CSB-EP312596PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP312597PUC
CSB-EP312597PUC
CSB-BP312597PUC
CSB-MP312597PUC
CSB-EP312597PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein phosphatase CheZ (cheZ) CSB-YP312598PUC
CSB-EP312598PUC
CSB-BP312598PUC
CSB-MP312598PUC
CSB-EP312598PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Recombination protein RecR (recR) CSB-YP312599PUC
CSB-EP312599PUC
CSB-BP312599PUC
CSB-MP312599PUC
CSB-EP312599PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP312600PUC
CSB-EP312600PUC
CSB-BP312600PUC
CSB-MP312600PUC
CSB-EP312600PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Bacteriohemerythrin (PA14_42860) CSB-YP312601PUC
CSB-EP312601PUC
CSB-BP312601PUC
CSB-MP312601PUC
CSB-EP312601PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Trigger factor (tig) CSB-YP312602PUC
CSB-EP312602PUC
CSB-BP312602PUC
CSB-MP312602PUC
CSB-EP312602PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable RNA methyltransferase PA14_40730 (PA14_40730) CSB-YP312603PUC
CSB-EP312603PUC
CSB-BP312603PUC
CSB-MP312603PUC
CSB-EP312603PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Pyrroloquinoline-quinone synthase (pqqC) CSB-YP312604PUC
CSB-EP312604PUC
CSB-BP312604PUC
CSB-MP312604PUC
CSB-EP312604PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Trans-aconitate 2-methyltransferase (tam) CSB-YP312605PUC
CSB-EP312605PUC
CSB-BP312605PUC
CSB-MP312605PUC
CSB-EP312605PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Translation initiation factor IF-1 (infA) CSB-YP312606PUC
CSB-EP312606PUC
CSB-BP312606PUC
CSB-MP312606PUC
CSB-EP312606PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L20 (rplT) CSB-YP312607PUC
CSB-EP312607PUC
CSB-BP312607PUC
CSB-MP312607PUC
CSB-EP312607PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Elongation factor P (efp) CSB-YP312608PUC
CSB-EP312608PUC
CSB-BP312608PUC
CSB-MP312608PUC
CSB-EP312608PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L32 (rpmF) CSB-YP312609PUC
CSB-EP312609PUC
CSB-BP312609PUC
CSB-MP312609PUC
CSB-EP312609PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP312610PUC
CSB-EP312610PUC
CSB-BP312610PUC
CSB-MP312610PUC
CSB-EP312610PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Cytidylate kinase (cmk) CSB-YP312611PUC
CSB-EP312611PUC
CSB-BP312611PUC
CSB-MP312611PUC
CSB-EP312611PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable septum site-determining protein MinC (minC) CSB-YP312612PUC
CSB-EP312612PUC
CSB-BP312612PUC
CSB-MP312612PUC
CSB-EP312612PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0276 protein PA14_21580 (PA14_21580) CSB-YP312613PUC
CSB-EP312613PUC
CSB-BP312613PUC
CSB-MP312613PUC
CSB-EP312613PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Aliphatic amidase (amiE) CSB-YP312614PUC
CSB-EP312614PUC
CSB-BP312614PUC
CSB-MP312614PUC
CSB-EP312614PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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