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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces pastorianus Homoserine O-acetyltransferase (MET2) CSB-YP312390SAJ
CSB-EP312390SAJ
CSB-BP312390SAJ
CSB-MP312390SAJ
CSB-EP312390SAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Serine acetyltransferase (cysE) CSB-YP312391BRJ
CSB-EP312391BRJ
CSB-BP312391BRJ
CSB-MP312391BRJ
CSB-EP312391BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative TrmH family tRNA/rRNA methyltransferase YacO (yacO) CSB-YP312392BRJ
CSB-EP312392BRJ
CSB-BP312392BRJ
CSB-MP312392BRJ
CSB-EP312392BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Puccinia graminis Protein USP1 (USP1) CSB-YP312393PWW
CSB-EP312393PWW
CSB-BP312393PWW
CSB-MP312393PWW
CSB-EP312393PWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Preprotein translocase subunit SecE (secE), partial CSB-YP312394BRJ
CSB-EP312394BRJ
CSB-BP312394BRJ
CSB-MP312394BRJ
CSB-EP312394BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YPR078C (YPR078C) CSB-YP312395SVG
CSB-EP312395SVG
CSB-BP312395SVG
CSB-MP312395SVG
CSB-EP312395SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial distribution and morphology protein 36 (MDM36), partial CSB-YP312396SVG
CSB-EP312396SVG
CSB-BP312396SVG
CSB-MP312396SVG
CSB-EP312396SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Alcohol-sensitive RING finger protein 1 (ASR1) CSB-YP312397
CSB-EP312397
CSB-BP312397
CSB-MP312397
CSB-EP312397-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coenzyme F420 hydrogenase subunit alpha (frhA) CSB-YP312398MSD
CSB-EP312398MSD
CSB-BP312398MSD
CSB-MP312398MSD
CSB-EP312398MSD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nuclear migration protein NUM1 (NUM1), partial CSB-YP312399SVG
CSB-EP312399SVG
CSB-BP312399SVG
CSB-MP312399SVG
CSB-EP312399SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colletotrichum lindemuthianum Endopolygalacturonase 1 (PG1) CSB-YP312400CXC
CSB-EP312400CXC
CSB-BP312400CXC
CSB-MP312400CXC
CSB-EP312400CXC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toluene-4-monooxygenase system protein A (tmoA) CSB-YP312401PUO
CSB-EP312401PUO
CSB-BP312401PUO
CSB-MP312401PUO
CSB-EP312401PUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Probable transposase for insertion sequence element IS702 CSB-YP312402FCY
CSB-EP312402FCY
CSB-BP312402FCY
CSB-MP312402FCY
CSB-EP312402FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida maltosa Acyl-coenzyme A oxidase 2 (POX2), partial CSB-YP312403CZJ
CSB-EP312403CZJ
CSB-BP312403CZJ
CSB-MP312403CZJ
CSB-EP312403CZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cymbidium mosaic virus Uncharacterized 12.4 kDa protein CSB-YP312404CVM
CSB-EP312404CVM
CSB-BP312404CVM
CSB-MP312404CVM
CSB-EP312404CVM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Shikimate kinase, chloroplastic CSB-YP312405SZY
CSB-EP312405SZY
CSB-BP312405SZY
CSB-MP312405SZY
CSB-EP312405SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Type II secretion system protein J (xcpW) CSB-YP312406EZX
CSB-EP312406EZX
CSB-BP312406EZX
CSB-MP312406EZX
CSB-EP312406EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Creopus spinulosus Frequency clock protein (FRQ), partial CSB-YP312407CRU
CSB-EP312407CRU
CSB-BP312407CRU
CSB-MP312407CRU
CSB-EP312407CRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Pectate lyase plyB (plyB) CSB-YP312408EKF
CSB-EP312408EKF
CSB-BP312408EKF
CSB-MP312408EKF
CSB-EP312408EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dianthus caryophyllus Putative beta-galactosidase (CARSR12), partial CSB-YP312409DKA
CSB-EP312409DKA
CSB-BP312409DKA
CSB-MP312409DKA
CSB-EP312409DKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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