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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactococcus lactis subsp. cremoris UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP311722LBE
CSB-EP311722LBE
CSB-BP311722LBE
CSB-MP311722LBE
CSB-EP311722LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris CCA-adding enzyme (cca) CSB-YP311723LBE
CSB-EP311723LBE
CSB-BP311723LBE
CSB-MP311723LBE
CSB-EP311723LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP311724LBE
CSB-EP311724LBE
CSB-BP311724LBE
CSB-MP311724LBE
CSB-EP311724LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Tryptophan synthase alpha chain (trpA) CSB-YP311725LBE
CSB-EP311725LBE
CSB-BP311725LBE
CSB-MP311725LBE
CSB-EP311725LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Glutamate racemase (murI) CSB-YP311726LBE
CSB-EP311726LBE
CSB-BP311726LBE
CSB-MP311726LBE
CSB-EP311726LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP311727LBE
CSB-EP311727LBE
CSB-BP311727LBE
CSB-MP311727LBE
CSB-EP311727LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP311728LBE
CSB-EP311728LBE
CSB-BP311728LBE
CSB-MP311728LBE
CSB-EP311728LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris tRNA pseudouridine synthase B (truB) CSB-YP311729LBE
CSB-EP311729LBE
CSB-BP311729LBE
CSB-MP311729LBE
CSB-EP311729LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UPF0042 nucleotide-binding protein LACR_1047 (LACR_1047) CSB-YP311730LBE
CSB-EP311730LBE
CSB-BP311730LBE
CSB-MP311730LBE
CSB-EP311730LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP311731LBE
CSB-EP311731LBE
CSB-BP311731LBE
CSB-MP311731LBE
CSB-EP311731LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP311732LBE
CSB-EP311732LBE
CSB-BP311732LBE
CSB-MP311732LBE
CSB-EP311732LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Hydrogenase expression/formation protein hupG (hupG) CSB-YP311733RLC
CSB-EP311733RLC
CSB-BP311733RLC
CSB-MP311733RLC
CSB-EP311733RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Iron sulfur cluster assembly protein 1, mitochondrial (ISU1) CSB-YP311734SVG
CSB-EP311734SVG
CSB-BP311734SVG
CSB-MP311734SVG
CSB-EP311734SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Oxaloacetate decarboxylase alpha chain (oadA1), partial CSB-YP311735SXB
CSB-EP311735SXB
CSB-BP311735SXB
CSB-MP311735SXB
CSB-EP311735SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Didelphis marsupialis virginiana Alpha-1-antiproteinase CSB-YP311736DKL
CSB-EP311736DKL
CSB-BP311736DKL
CSB-MP311736DKL
CSB-EP311736DKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 6 CSB-YP311737RON
CSB-EP311737RON
CSB-BP311737RON
CSB-MP311737RON
CSB-EP311737RON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Down-regulator of invasive growth 1 (DIG1) CSB-YP311738SVG
CSB-EP311738SVG
CSB-BP311738SVG
CSB-MP311738SVG
CSB-EP311738SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Uncharacterized protein YPL039W (YPL039W) CSB-YP311739SVG
CSB-EP311739SVG
CSB-BP311739SVG
CSB-MP311739SVG
CSB-EP311739SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Styryl dye vacuolar localization protein 3 (SVL3), partial CSB-YP311740SVG
CSB-EP311740SVG
CSB-BP311740SVG
CSB-MP311740SVG
CSB-EP311740SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Leucine--tRNA ligase (leuS), partial CSB-YP311741LBE
CSB-EP311741LBE
CSB-BP311741LBE
CSB-MP311741LBE
CSB-EP311741LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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