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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Popillia japonica Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 CSB-YP311762PPQ
CSB-EP311762PPQ
CSB-BP311762PPQ
CSB-MP311762PPQ
CSB-EP311762PPQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sciara coprophila Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2, partial CSB-YP311763SDT
CSB-EP311763SDT
CSB-BP311763SDT
CSB-MP311763SDT
CSB-EP311763SDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urease accessory protein UreD (ureD) CSB-YP311764ENL
CSB-EP311764ENL
CSB-BP311764ENL
CSB-MP311764ENL
CSB-EP311764ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris N-acetyldiaminopimelate deacetylase (LACR_0294) CSB-YP311765LBE
CSB-EP311765LBE
CSB-BP311765LBE
CSB-MP311765LBE
CSB-EP311765LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris S-ribosylhomocysteine lyase (luxS) CSB-YP311766LBE
CSB-EP311766LBE
CSB-BP311766LBE
CSB-MP311766LBE
CSB-EP311766LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP311767LBE
CSB-EP311767LBE
CSB-BP311767LBE
CSB-MP311767LBE
CSB-EP311767LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris UPF0297 protein LACR_0137 (LACR_0137) CSB-YP311768LBE
CSB-EP311768LBE
CSB-BP311768LBE
CSB-MP311768LBE
CSB-EP311768LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ribonuclease P protein component (rnpA) CSB-YP311769LBE
CSB-EP311769LBE
CSB-BP311769LBE
CSB-MP311769LBE
CSB-EP311769LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toluene-4-monooxygenase electron transfer component (tmoF) CSB-YP311770PUO
CSB-EP311770PUO
CSB-BP311770PUO
CSB-MP311770PUO
CSB-EP311770PUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable ATP-dependent RNA helicase prh1 (prh1), partial CSB-YP311771SXV
CSB-EP311771SXV
CSB-BP311771SXV
CSB-MP311771SXV
CSB-EP311771SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein MSH-B (msxb) CSB-YP311772DIL
CSB-EP311772DIL
CSB-BP311772DIL
CSB-MP311772DIL
CSB-EP311772DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Comitin (comA) CSB-YP311773DKK
CSB-EP311773DKK
CSB-BP311773DKK
CSB-MP311773DKK
CSB-EP311773DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Eukaryotic initiation factor iso-4F subunit p82-34, partial CSB-YP311774TQN
CSB-EP311774TQN
CSB-BP311774TQN
CSB-MP311774TQN
CSB-EP311774TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP311775LBE
CSB-EP311775LBE
CSB-BP311775LBE
CSB-MP311775LBE
CSB-EP311775LBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Glucosamine-6-phosphate deaminase (nagB) CSB-YP311776LAN
CSB-EP311776LAN
CSB-BP311776LAN
CSB-MP311776LAN
CSB-EP311776LAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus RNA polymerase sigma-54 factor (rpoN) CSB-YP311777DQO
CSB-EP311777DQO
CSB-BP311777DQO
CSB-MP311777DQO
CSB-EP311777DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Fructokinase (frk) CSB-YP311778ZBM
CSB-EP311778ZBM
CSB-BP311778ZBM
CSB-MP311778ZBM
CSB-EP311778ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea atroviridis Alkaline proteinase (prb1) CSB-YP311779HRP
CSB-EP311779HRP
CSB-BP311779HRP
CSB-MP311779HRP
CSB-EP311779HRP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial zinc maintenance protein 1, mitochondrial (MZM1) CSB-YP311780SVG
CSB-EP311780SVG
CSB-BP311780SVG
CSB-MP311780SVG
CSB-EP311780SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Group 1 truncated hemoglobin CSB-YP311781TLU
CSB-EP311781TLU
CSB-BP311781TLU
CSB-MP311781TLU
CSB-EP311781TLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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