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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus thermophilus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP311904SNF
CSB-EP311904SNF
CSB-BP311904SNF
CSB-MP311904SNF
CSB-EP311904SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP311905SNF
CSB-EP311905SNF
CSB-BP311905SNF
CSB-MP311905SNF
CSB-EP311905SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus UPF0374 protein STER_0421 (STER_0421) CSB-YP311906SNF
CSB-EP311906SNF
CSB-BP311906SNF
CSB-MP311906SNF
CSB-EP311906SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus Urease accessory protein UreF (ureF) CSB-YP311907SNF
CSB-EP311907SNF
CSB-BP311907SNF
CSB-MP311907SNF
CSB-EP311907SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP311908SNF
CSB-EP311908SNF
CSB-BP311908SNF
CSB-MP311908SNF
CSB-EP311908SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP311909SNF
CSB-EP311909SNF
CSB-BP311909SNF
CSB-MP311909SNF
CSB-EP311909SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus thermophilus Putative competence-damage inducible protein (cinA) CSB-YP311910SNF
CSB-EP311910SNF
CSB-BP311910SNF
CSB-MP311910SNF
CSB-EP311910SNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Thiamine-phosphate synthase (thiE) CSB-YP311911LAL
CSB-EP311911LAL
CSB-BP311911LAL
CSB-MP311911LAL
CSB-EP311911LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis L-arabinose isomerase (araA) CSB-YP311912LAL
CSB-EP311912LAL
CSB-BP311912LAL
CSB-MP311912LAL
CSB-EP311912LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis 30S ribosomal protein S10 (rpsJ) CSB-YP311913LAL
CSB-EP311913LAL
CSB-BP311913LAL
CSB-MP311913LAL
CSB-EP311913LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP311914LAL
CSB-EP311914LAL
CSB-BP311914LAL
CSB-MP311914LAL
CSB-EP311914LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Xanthine phosphoribosyltransferase (xpt) CSB-YP311915LAL
CSB-EP311915LAL
CSB-BP311915LAL
CSB-MP311915LAL
CSB-EP311915LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP311916LAL
CSB-EP311916LAL
CSB-BP311916LAL
CSB-MP311916LAL
CSB-EP311916LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis D-ribose pyranase (rbsD) CSB-YP311917LAL
CSB-EP311917LAL
CSB-BP311917LAL
CSB-MP311917LAL
CSB-EP311917LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP311918LAL
CSB-EP311918LAL
CSB-BP311918LAL
CSB-MP311918LAL
CSB-EP311918LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP311919LAL
CSB-EP311919LAL
CSB-BP311919LAL
CSB-MP311919LAL
CSB-EP311919LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis ATP synthase gamma chain (atpG) CSB-YP311920LAL
CSB-EP311920LAL
CSB-BP311920LAL
CSB-MP311920LAL
CSB-EP311920LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Putative competence-damage inducible protein (cinA) CSB-YP311921LAL
CSB-EP311921LAL
CSB-BP311921LAL
CSB-MP311921LAL
CSB-EP311921LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis MutS2 protein (mutS2), partial CSB-YP311922LAL
CSB-EP311922LAL
CSB-BP311922LAL
CSB-MP311922LAL
CSB-EP311922LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus brevis Threonine--tRNA ligase (thrS), partial CSB-YP311923LAL
CSB-EP311923LAL
CSB-BP311923LAL
CSB-MP311923LAL
CSB-EP311923LAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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