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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Loxosceles deserta Sphingomyelin phosphodiesterase D LdSicTox-alpha-1 CSB-YP313532LQY
CSB-EP313532LQY
CSB-BP313532LQY
CSB-MP313532LQY
CSB-EP313532LQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hadronyche formidabilis Omega-hexatoxin-Hf1a CSB-YP313533HAA
CSB-EP313533HAA
CSB-BP313533HAA
CSB-MP313533HAA
CSB-EP313533HAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate-binding protein pstS 2 (pstS2) CSB-YP313534FMW
CSB-EP313534FMW
CSB-BP313534FMW
CSB-MP313534FMW
CSB-EP313534FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine carbamoyltransferase, mitochondrial (arg1) CSB-YP313535DPC
CSB-EP313535DPC
CSB-BP313535DPC
CSB-MP313535DPC
CSB-EP313535DPC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine hemagglutinating encephalomyelitis virus Truncated non-structural protein of 4.9 kDa CSB-YP313536PPX
CSB-EP313536PPX
CSB-BP313536PPX
CSB-MP313536PPX
CSB-EP313536PPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Envelope small membrane protein (E), partial CSB-YP313537BJI1
CSB-EP313537BJI1
CSB-BP313537BJI1
CSB-MP313537BJI1
CSB-EP313537BJI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Envelope small membrane protein (E), partial CSB-YP313538MJY1
CSB-EP313538MJY1
CSB-BP313538MJY1
CSB-MP313538MJY1
CSB-EP313538MJY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endoglucanase C (celC) CSB-YP313539CMO
CSB-EP313539CMO
CSB-BP313539CMO
CSB-MP313539CMO
CSB-EP313539CMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heparin-binding hemagglutinin (hbhA) CSB-YP313540MVH
CSB-EP313540MVH
CSB-BP313540MVH
CSB-MP313540MVH
CSB-EP313540MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. lactis Di-/tripeptide transporter (dtpT), partial CSB-YP313541LNG
CSB-EP313541LNG
CSB-BP313541LNG
CSB-MP313541LNG
CSB-EP313541LNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycosa singoriensis M-lycotoxin-Ls3b CSB-YP313542LRO
CSB-EP313542LRO
CSB-BP313542LRO
CSB-MP313542LRO
CSB-EP313542LRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Negative regulator of flagellin synthesis (flgM) CSB-YP313543YAQ
CSB-EP313543YAQ
CSB-BP313543YAQ
CSB-MP313543YAQ
CSB-EP313543YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato golden mosaic virus Protein AC4 (AC4, AL4) CSB-YP313544TGE
CSB-EP313544TGE
CSB-BP313544TGE
CSB-MP313544TGE
CSB-EP313544TGE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica ATP synthase epsilon chain, chloroplastic (atpE) CSB-YP313546OFG
CSB-EP313546OFG
CSB-BP313546OFG
CSB-MP313546OFG
CSB-EP313546OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Chloroplast envelope membrane protein (cemA), partial CSB-YP313547OCO1
CSB-EP313547OCO1
CSB-BP313547OCO1
CSB-MP313547OCO1
CSB-EP313547OCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa Uncharacterized protein ycf73 (ycf73-A) CSB-YP313548OCO
CSB-EP313548OCO
CSB-BP313548OCO
CSB-MP313548OCO
CSB-EP313548OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP313549OCO
CSB-EP313549OCO
CSB-BP313549OCO
CSB-MP313549OCO
CSB-EP313549OCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus striatus Mu-conotoxin SIIIC CSB-YP313550DWI
CSB-EP313550DWI
CSB-BP313550DWI
CSB-MP313550DWI
CSB-EP313550DWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem I iron-sulfur center (psaC) CSB-YP313551OFG
CSB-EP313551OFG
CSB-BP313551OFG
CSB-MP313551OFG
CSB-EP313551OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP313552OFF1
CSB-EP313552OFF1
CSB-BP313552OFF1
CSB-MP313552OFF1
CSB-EP313552OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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